摘要
【目的】从疑似鸭疫里默氏杆菌病的病死雏鹅分离病原菌进行鉴定。【方法】根据细菌培养特性、生化特性、动物试验、血清型鉴定及分子生物学特性对分离菌株进行鉴定。【结果】分离菌株为革兰氏阴性菌,不发酵糖类和醇类,尿素酶试验和氧化酶还原试验为阳性,致病,不同分离株的16S rRNA基因经多重序列比对分析,结果显示鹅源分离株与鸭源鸭疫里默氏杆菌处于同一进化支上,与鸡源鸭疫里默氏杆菌进化关系稍远,血清型鉴定为1型。【结论】分离菌株为血清1型的鸭疫里默氏杆菌,对鸭和鹅均有高致病性,自家疫苗能够较好地保护雏鹅。
[Objective] To identify pathogenic bacteria isolated from dead goslings suspected Riemerella anatipestifer disease. [Methods] The isolated bacterial strain was subjected to examine by the cultural performances, biochemical properties, animal text, serological identification and biological features. [Results] The results showed that the isolated strain was identified as gram-negative bacteria, which could not ferment and alcohols, and it was positive of ureolysis test and oxidase test. Pathogenic. The multiple sequence alignment from different isolated stains 16S rRNA genes showed, the strain in this study was at the same evolution branches with the Riemerella anatipestifer isolated from ducks, but it was in little relation to the Riemerella anatipestifer isolated from chicken. It belongs to the serotype I of Riemerella anatipestifer. [Conclusion] The isolated bacterium belongs to the serotype I of Riemerella anatipestifer, which was highly pathogenic to the ducking and goosing, and the self-development vaccine could protect the goslings effectively against to be infected by Riemerella anatipestifer.
出处
《微生物学通报》
CAS
CSCD
北大核心
2012年第12期1755-1761,共7页
Microbiology China
基金
广东省科技厅国际合作专项(No.2008A050200015)
广东省自然科学基金项目(No.9251064001000010)
