摘要
目的:建立知柏地黄丸中盐酸小檗碱和盐酸巴马汀的含量测定,控制知柏地黄丸的质量。方法:采用高效液相色谱法,在Welch Materials XB-C18(5μm,250 mm×4.6 mm)色谱柱上,以乙腈为流动相A,以0.1%三氟乙酸为流动相B,梯度洗脱,检测波长为345 nm,柱温25℃。结果:盐酸小檗碱在3.78～37.8μg.mL-1的浓度范围内具有良好的线性关系,盐酸巴马汀在2.65～26.5μg.mL-1的浓度范围内具有良好的线性关系。盐酸小檗碱和盐酸巴马汀的平均回收率(n=6)分别为97.6%(RSD=1.1%)和99.5%(RSD=0.2%)。结论:用本文方法检验了268批知柏地黄丸样品,发现41批混淆使用了关黄柏。
Objective：To develop an HPLC method for determination of berberine hydrochloride and palmatine hydrochloride in Zhibai Dihuang pills, so as to provide a reference for the quality control of Zhibai Dihuang pills. Method：The separation was performed on a Welch Materials XB -C18 （250 mm× 4.6 mm,5 μm） column with the mobile phase consisting of acetonitrile （A） and water containing 0. 1% trifluoroacetic acid （B） in gradient elution. The detection wavelength was set at 345 nm,and the column temperature was 25 ℃. Result： The halibration curves were linear within the range of 3.78 -37.8 μg · mL^-1for berberine hydroehloride and 2.65 -26.50 μg · mL^-1 for palmatine hydrochloride. The average recoveries were 97.6% （ RSD = 1.1% ） for berberine hydrochloride and 99.5% （RSD =0.2% ）for palmatine hydrochloride. Conclusion：： There were 41 batches of samples confused with Phellodendri Amurensis Cortex and Phellodendri Chinensis Cortex in 268 batches of Zhibai Dihuang pills by use of this paper method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第12期2198-2202,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
知柏地黄丸
黄柏
关黄柏
盐酸小檗碱
盐酸巴马汀
含量测定
质量控制
Zhibai Dihuang pills
Phellodendri Amurensis Cortex
Phellodendri Chinensis Cortex
berberine hydro- chloride
palmatine hydrochloride
content determination
quality control
