摘要
目的观察天麻素预处理对大鼠心肌缺血再灌注损伤的保护作用并探讨其可能机制。方法健康SD大鼠,随机分为假手术组、生理盐水组、低浓度天麻素组、中浓度天麻素组、高浓度天麻素组和维拉帕米组,应用结扎大鼠左冠状动脉前降支30 min,再灌注120 min的方法,从心律失常发生率、心肌组织形态学、血清肌酸激酶、乳酸脱氢酶、超氧化物歧化酶和丙二醛含量的变化来观察天麻素预处理对大鼠心肌缺血再灌注损伤的保护作用,并进一步通过激光共聚焦Ca2+成像技术探讨天麻素对细胞钙库操控的钙内流的影响。结果天麻素预处理后能降低大鼠心肌缺血再灌注损伤时心律失常发生率,改善心肌组织形态学的损伤性变化,提高心肌超氧化物歧化酶含量,降低乳酸脱氢酶、血清肌酸激酶和丙二醛含量,并对非选择性阳离子通道具有一定的抑制作用。结论天麻素预处理对大鼠心肌缺血再灌注损伤有明显的保护作用,可能与其增强超氧化物歧化酶清除氧自由基能力,抑制细胞内钙超载有关。
OBJECTIVE To observe the effect of gastrodin preconditioning on the myocardial ischemia reperfusion injury （MIRI） in rats and to study the mechanism. METHODS SD rats were randomized into six groups including sham group, NS group, gastrodin groups of low middle and high concentrations and verapamil group. The rats were treated with gastrodin via intragastric administration for one week. Then the rats were treated by closing the left anterior descending coronary artery for 30 min and then releasing it for 2 h under anesthesia. The changes of the arrhythmia, myocardial histological structure and serological tests including SOD, LDH, CK-MB and MDA were examined. Furthermore, the effect of gastrodin on store-operated Ca^2＋influx was also investigated. RESULTS It was found that gastrodin significantly enhanced SOD production and suppressed the incidences of arrhythmia and LDH, CK-MB and MDA concentrations. Additionally, gastrodin inhibited store-operated Ca^2＋ influx. CONCLUSION Gastrodin has protective effect on myo- cardium against ischemia reperfusion injury in rats through several pathways including cleaning oxygen radicals and reducing the over- load ofCa^2＋.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第23期1905-1910,共6页
Chinese Pharmaceutical Journal
基金
安徽省杰出青年基金资助项目(1108085J11)
安徽医科大学博士科研资助基金(XJ200913)
校级优秀青年教师后备人选基金资助
关键词
天麻素
缺血再灌注损伤
心律失常
钙超载
肌酸激酶
乳酸脱氢酶
超氧化物歧化酶
丙二醛
KEY WORDS ： gastrodin
ischemia-reperfusion injury
arrhythmia
overload ofCa^2＋
creatine kinase
lactate dehydrogenase
super-oxide dismutase
malonaldehyde
