摘要
为了研究红豆杉细胞反应器悬浮培养的流变特性,利用旋转粘度计测定了反应器悬浮培养过程悬浮液的表观粘度,发现红豆杉悬浮细胞培养的流变性可以用幂律模型来描述,不同培养时期培养液均属于假塑性非牛顿流体;基于实验数据,通过线性回归,得到了不同培养时期培养液的流变本构方程、稠度系数和流动特性指数,建立了反应器中培养液表观粘度与细胞浓度的线性关系式和单位细胞浓度培养液表观粘度与细胞聚集体组成的线性关系式。这些结果可做为红豆杉细胞悬浮培养过程操作控制、反应器设计和放大的重要依据。
In this paper, rheological properties of Taxus media cells suspension cultures in a bioreactor were investiga ted. The rheological equations were obtained by regression analysis. The results demonstrated that the suspension cul tures broth of Taxus media cells at different fermentation time exhibited pseudoplastic non-Newtonian fluid, the broth apparent viscosity increased linearly with the increase in cell concentration and there was the linear functional relations be tween the apparent viscosity and the percentage aggregation. The pseudoplasticity and consistency coefficient of the broth increased with time from lag phase to stationary phase of cell growth. Subsequently, both appeared to decrease. At the same time the flow behavior index decreased with time from lag phase to stationary phase and increased during decline phase of cell growth. The results should be helpful in operation control, reactor design and amplification of Taxus media cell suspension cultures in a bioreactor.
出处
《武汉理工大学学报》
CAS
CSCD
北大核心
2012年第11期36-40,共5页
Journal of Wuhan University of Technology
基金
国家科技支撑计划项目(2008BAI6 3B04)
关键词
红豆杉
悬浮培养
流变特性
表观粘度
反应器
Taxus media
suspension culture
rheological property apparent viscosity bioreactor
