摘要
Objective： The aim of our study was to discovery the different cell cycle arrest effect after different densities HeLa cells treated with Trichostatin A （TSA）. In addition, this study would find some important relationship between cycle arrest effect and UV absorption spectrum of cell. Methods： 0.2 IJM TSA was applied to act on HeLa cells of different density. Then, the cycle arrest effect and UV absorption spectrum of cells were investigated, which provide support to analyze the effect of TSA on cancer cells. Results： Cell cycle arrest effect in G0/G1 of the lower density cells was more obvious than that in other groups. The other discovery in this work was that the cellular UV absorption value was higher when the density of cultured cell was lower. Conclusion： This experiment would guide the clinical study on early or late stage cancer patients in the future. On the other hand, this work indicates when cells were arrested in G0/G1 phase, the cellular absorption value increased at the same time, so UV absorption spectrum could characterize the change of cell cycle.
基金
Supported by a grant from the Education Office of Henan Province in China (No.12A140013)
