摘要
microRNAs are single-stranded,non-coding RNAs that regulate gene expression.The microRNA-16 family has been reported to be involved in cell-cycle regulation,which could also downregulate expression of multiple pro-proliferation genes.The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate (MMS) treatment.This further resulted in downregulation of a target protein CDC25A,whereas miR-16 expression did not increase when HeLa cells were arrested during the MMS-treated G0/G1 or G2/M phase.Furthermore,when HeLa cells were arrested during S-phase with hydroxyurea treatment,miR-16 expression did not increase.These results suggest that expression levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.
microRNAs are single-stranded, non-coding RNAs that regulate gene expression. The microRNA-16 family has been reported to be involved in cell-cycle regulation, which could also downregulate expression of multiple pro-proliferation genes. The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate （MMS） treatment. This further resulted in downregulation of a target protein CDC25A, whereas miR-16 expres- sion did not increase when HeLa ceils were arrested during the MMS-treated G0/G1 or G2/M phase. Furthermore, when HeLa cells were arrested during S-phase with hydroxyurea treatment, miR-16 expression did not increase. These results suggest that expres- sion levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.
基金
supported by the National Key Basic Research and Development Program of China (2006CB503900)
National Natural Science Foundation of China (30821065)
Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX1-YW-02 and KJCX2-YW-M15)
Science and Technology Commission of Shanghai Municipality (07dz05907)
