摘要
目的建立清毒安肾胶囊高效液相色谱指纹图谱。方法色谱条件为Ultimate XB-C18(3.0 mm×150 mm,3μm)色谱柱,以乙腈(A)-0.04%磷酸溶液(B)为流动相,非线性梯度洗脱,洗脱程序如下:0～10 min(2%→18%A),10～20 min(18%A),20～55 min(18%→30%A),55～90 min(30%→88%A);检测波长:203 nm、260 nm;流速:0.6 mL.min-1;柱温:30℃。结果该指纹图谱中,确定了34个共有色谱峰,可检出清毒安肾胶囊中9味药材;并采用RRLC/MS/MS及HPLC-PDA鉴定了指纹图谱中20个化学成分。结论该方法准确可靠,重复性好,为清毒安肾胶囊的质量控制提供了有效手段。
Objective To establish an HPLC fingerprint of Qingdu Anshen capsules. Methods The fingerprint was obtained on an Ultimate XB-C18 column （3.0 mm ×150 mm, 3 μm） with the solvent system composed of acetonitrile （A）-0.04% phos- phoric acid （B）. The elution profile （nonlinear gradient） was： 0 -- 10 min （2%→18%A）, 10 -- 20 min （18%A）, 20 -- 55 min （18%→30%A）, and 55 -- 90 min （30%→88%A）. The detective wavelengths were 203 nm and 260 nm. The column temperature was 30℃and the flow rate was 0.6 mL · min^- 1. Results In the established fingerprint, 34 common chromat-egraphic peaks covering 9 medicinal materials were detected, and 20 chemical compounds were identified by RRLC/MS/MS and PDA spectra. Conclusion The method can be used for the quality control of Qingdu Anshen capsules with great preci- sion and good reproducibility.
出处
《中南药学》
CAS
2012年第11期847-850,共4页
Central South Pharmacy
基金
广东省中国科学院全面战略合作项目(编号:2009B091300126)
