摘要
目的:对中国被毛孢菌丝体(Hirsutella sinensis mycelium,简称HSM)治疗特发性肺纤维化(IPF)大鼠的作用及其作用机制进行研究和探讨。方法:取40只Wistar大鼠分为5组:正常对照组、模型对照组、HSM高、低剂量组(1.0,0.5 g.kg-1)、阳性对照组(氢化可的松10 mg.kg-1)。除对照组大鼠外,大鼠气管注入5 mg.kg-1博莱霉素进行肺纤维化造模,连续给药28 d后,对各组大鼠肺功能、肺组织羟脯氨酸、细胞因子和病理学进行观察。结果:IPF大鼠给予中国被毛孢菌丝体后,HSM 0.5,1.0 g.kg-1能显著降低大鼠肺脏指数和羟脯氨酸含量(P<0.01),同时能明显改善肺功能、肺组织肺泡炎症和肺纤维化程度(P<0.05,P<0.01);1.0 g.kg-1HSM能显著降低大鼠肺组织中TNF-α,IL-1β,TGF-β1蛋白表达(P<0.01),同时能升高肺组织中IFN-γ蛋白表达(P<0.05)。结论:中国被毛孢菌丝体对大鼠肺纤维化有较好的治疗作用,其治疗作用机制与调节TNF-α,IL-1β,TGF-β1和IFN-γ失衡有关。
Objective： To study and discuss the effect and mechanism of Hirsutella sinensis mycelium （HSM） on idiopathic pulmonary fibrosis in rats. Method： Forty Wistar rats were divided into five groups： the normal control group, the model control group, the high-dose group （1.0 g·kg^-1HSM）, the low-dose group （0. 5 g·kg^-1 HSM）, and the positive control group （10 mg·kg^-1 bydrocortisone）. In addition to rats in the normal control group, the pulmonary fibrosis model was established by injecting 5 mg · kg^-1 bleomycin into rat tracheas for consecutively 28 days, in order to observe their lung function, lung tissue hydroxyprohne, cyto- kines and pathology. Result： After rats were administered with HSM, 0. 5 g·kg^-1 and 1.0 g·kg^-1 HSM could significantly decrease lung index and hydroxyproline content （ P 〈 0. 01）, while notably improving pulmonary function, alveolus inflammation and fibrosis degree （P 〈0. 05, P 〈0.01 ） ; 1.0 g·kg^-1 HSM could decrease significantly protein expressions of TNF-α, IL-1β and TGF-β1 in lung tissues, while increasing significantly protein expressions of IFN-T （P 〈 0.05）. Conclusion： HSM have better effect in treating idiopathic pulmonary fibrosis in rats. Its treatment effect and mechanism are related to the regulation of TNF-α, IL-1β and TGF-β1 and IFN-γ imbalance.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第23期3618-3623,共6页
China Journal of Chinese Materia Medica
基金
浙江省自然科学基金项目(Y2110727)
浙江省中医药科学研究基金计划项目(2011ZA021)
