摘要
目的:从细胞周期蛋白激酶抑制因子(CKI)途径入手探讨鸡血藤黄酮类有效部位(SSCE)对人肺癌A549细胞的周期阻滞作用的分子机制。方法:采用RT-PCR法检测人肺腺癌A549细胞中p21、p27 mRNA表达;应用Western Blotting法测定人肺腺癌A549细胞中p21、p27蛋白表达。结果:160mg/L SSCE作用于人肺腺癌A549细胞24h后,细胞内p21 mRNA表达较对照组增加(P<0.01);p21蛋白含量较对照组高(P<0.01),而细胞内p27 mRNA表达及蛋白含量与对照组比较差异无统计学意义;160mg/L SSCE作用人肺腺癌A549细胞48h后,细胞内p21、p27 mRNA表达与对照组比较差异无统计学意义;细胞内p21、p27蛋白含量与对照组比较差异无统计学意义。结论:SSCE上调p21 mRNA转录水平及蛋白表达量是引起人肺腺癌A549细胞G2/M期细胞周期阻滞的机制之一;p21、p27对SSCE引起的人肺腺癌A549细胞G0/G1期阻滞无直接作用。
Objective： To study the effects of spatholobus suberectus flavonoids（SSCE） on cell cycle arrest and its molecular mechanism based on Cyclin-dependent kinase inhibitor（CKI） in human lung adenocarcinoma A549 cells.Methods： The expression of p21,p27 mRNA in human lung adenocarcinoma A549 cells was detected by RT-PCR method,expression of p21,p27 protein in human lung adenocarcinoma A549 cells was determined by Western Blotting method.Results： Expression of p21 mRNA in 160mg/L SSCE-24h-treated group was significantly higher than that of control group（P0.01）;content of p21 protein in 160mg/L SSCE-24h-treated group increased compared with control group,the level of p27 mRNA and protein in human lung adenocarcinoma A549 cells was with no difference to control group,expression of p21,p27 mRNA in 160mg/L SSCE-48h-treated group was with no difference to control group;content of p21,p27 protein in 160mg/L SSCE-48h-treated group was with no difference to control group.Conclusion： Up-regulating p21 mRNA transcription and protein expression in A549 cells by SSCE was one of the molecure mechanism in G1 phase arrest.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第12期3217-3220,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
北京市教育委员会科技计划面上项目(No.KM200810025008)~~
