摘要
目的探讨HLA-G基因3'非翻译区14bp插入/缺失多态性与不明原因复发性流产患者的相关性。方法选择343名不明原因复发性流产患者,按照不同流产次数分为流产2次组(n=152),流产3次组(n=132),流产4次及以上组(n=59);268名正常妊娠妇女作为健康对照组。采用聚合酶链反应(PCR)及8%非变性聚丙烯酰胺凝胶电泳分离技术检测HLA-G基因14bp插入/缺失多态性位点在不明原因复发性流产患者及正常妊娠组中的基因型频率分布。结果 HLA-G基因14bp插入/缺失多态性位点的基因型频率分布在流产4次及以上组与正常妊娠组差异有统计学意义(χ2=6.941,P=0.031),流产4次及以上组+14bp等位基因频率分布显著高于正常妊娠组(χ2=4.956,P=0.026,OR=1.573,95%CI:1.054～2.349)。结论 HLA-G 14 bp缺失多态性在维持正常的妊娠中可能有重要作用。
Objective To investigate the association between HLA -G gene 14 -bp insertion/deletion poly- morphism in 3 UTR and recurrent spontaneous abortions. Methods In this study, a total of 611 Chinese women were genotyped for the ＋ 14 - bp/14 - bp polymorphism, including 343 who had recurrent spontaneous abortions （ two miscarriages ： 152, three miscarriages ： 132, four or more miscarriages ： 59） , 268 women with normal fertility as controls. Results To our knowledge, this is the first report that Significant difference was observed in the distribution of ＋ 14 - bp/＋ 14 - bp genotype between controls and the recurrent abortion group with four or more abortions （ χ2 = 6. 941, P = 0.031 ）. The ＋ 14 - bp homozygote sequence was more prominent among those with recurrent spontaneous abortions （four or more recurrent miscarriages） in contrast to fertile control women （χ2= 4.956, P=0.026, 0R=1.573, 95%CI： 1.054-2.349）. Conclusion A 14 - bp insertion/deletion polymorphism in exon 8 has a possible role in HLA - G expression in certain cases of recurrent spontaneous abortions. However, additional studies are needed in this regard.
出处
《宁夏医科大学学报》
2012年第10期986-988,992,共4页
Journal of Ningxia Medical University
基金
国家自然科学基金(31100900)
