摘要
目的:探讨父源性HLA-G基因14bp缺失多态性与早发型重度子痫前期的相关性。方法:采用PCR方法,对中国地区汉族人群中40对早发型重度子痫前期父儿和50对正常晚孕者父儿进行HLA-G基因第8外显子14bp缺失多态性的等位基因分型,比较两组父亲之间和两组新生儿之间等位基因及基因型的频率分布,通过父/儿基因型配伍,比较两组间基因型配伍频率分布的差异。结果:①早发型重度子痫前期组新生儿HLA-G-14bp频率52.5%和-14bp/-14bp基因型频率显著低于对照组(P=0.024;P=0.010);②早发型重度子痫前期组父(-14bp/-14bp)/儿-14bp/-14bp基因型配伍频率显著低于对照组(P=0.013);③早发型重度子痫前期父亲组HLA-G 14bp缺失多态性的等位基因频率分布与正常对照组比较差异虽无统计学意义,但是有差异性趋势(P=0.051)。结论:父源性HLA-G基因14bp缺失多态性可能与早发型重度子痫前期的发病相关,父(-14bp/-14bp)/儿-14bp/-14bp基因型配伍可能会降低母亲患重度子痫前期的风险。
Objective：To investigate the association of 14bp insertion/deletion polymorphism of the HLA-G gene in father with early onset severe preeclampsia.Methods：HLA-G Exon 8 genotyping was performed by polymerase chain reaction（PCR） in 40 early onset severe preeclampsia patients＇ husbands and 50 normal late trimester pregnant women＇ husbands and their neonates,in which Chinese Han population.Respectively compare the distributions of the allele frequencies and genotype frequencies of the two groups,combined with father/ fetal genotype case-match to analyze the differences of the two group.Results：①The frequencies of HLA-G-14bp and-14bp/-14bp genotype in the fetus of early onset severe preeclampsia were significantly lower than control group（P=0.024;P=0.010）;②The frequencies of father（-14bp/-14bp）/fetal（-14bp/-14bp） of early onset severe preeclampsia was significantly lower than control group（P=0.013）;③No significant differences were observed between fathers the early onset severe preeclampsia and the control,but the distribution of HLA-G 14bp allele shown a significant trend（P=0.051）.Conclusion：HLA-G 14bp insertion/deletion polymorphism in father is possibly associated with early onset severe preeclampsia,father（-14bp/-14bp）/fetal（-14bp/-14bp） genotype may reduce the risk of the early onset severe preeclampsia.
出处
《中国妇幼保健》
CAS
北大核心
2012年第11期1710-1713,共4页
Maternal and Child Health Care of China
基金
国家自然科学基金项目〔30872316〕
