摘要
目的通过接种方式及小鼠品系的优化,建立细粒棘球蚴小鼠模型。方法 10周龄的昆明白小鼠和C57BL/6J小鼠各32只,分为4组,每组8只,分别给予人源细粒棘球蚴原头节悬液(2 000个/ml)0.1 ml直视下注射肝脏,腹腔接种人源细粒棘球蚴囊泡,腹腔注射无菌PBS溶液,并同时采用注射肝脏和腹腔接种囊泡等4种方式感染各组小鼠,通过腹部观察和病理检查分析肝脏包囊的生长情况、直径及其数量。结果双重感染180 d后,C57BL/6J小鼠的肝脏感染率达到37.50%,而对照组昆明白小鼠未见腹腔包囊和肝脏病灶,两组之间差异具有统计学意义(t=6.67,P<0.05)。结论利用双重感染方式(原头节直视下注射肝脏和囊泡腹腔接种)感染C57BL/6J小鼠,可用于建立肝囊型包虫病动物模型,为研究肝囊型包虫病的致病机制奠定基础。
Objective To establish mice model with cystic echinococcosis by optimizing infection methods and mouse strains.Methods Mice aged 10 weeks of 32 Kunming species and 32 C57BL/6J were divided into 4 groups respectively,and 8 mice in one group.The first group of both strains received hepatic injection with 0.1 ml injectable suspension of 2 000 protoscolexes/ml from human primary cystic echinococcosis lesion;The second group was peritoneally infected with vesicles from human primary CE;The third group was infected both with protoscolexes hepatic injection and vesicles infection peritoneally;control mice received 0.1 ml sterilized PBS.Growth,number and diameter of cysts in the livers were detected by pathology and vitro experiment.Results After double infection for 180 days,the successful infectious rate of C57BL/6J mice livers was 37.5%;while in the group of Kunming species mice with double infection,no peritoneal cysts or liver lesions were found.There was a significant difference in liver lesion number between double infectious C57BL/6J mice and Kunming species mice（t=6.67,P〈0.05）.Conclusions We find that double infection（protoscolexes hepatic injection and vesicles infection peritoneally） in C57BL/6J mice can be used to establish the cystic echinococcosis liver model,lay the foundation for the research of hepatic cystic echinococcosis pathogenic mechanism.
出处
《疾病预防控制通报》
2012年第5期1-4,7,共5页
Bulletin of Disease Control & Prevention(China)
基金
新疆维吾尔自治区包虫病基础医学重点实验室开放课题(XJDX0202-2008-03)
