摘要
[ Objective ] In order to get the standard substance of Jinggangmycin used in quality control, Jinggangmycin was purified from its original pesticide and its content was determined, t Method] Mixed solvents were selected to recrystallize Jinggangmycin （content 62% ）. Phenol-sulfuric acid colofirnetric method was adopted to determine its content, and the effects of different determination conditions on the content results were also studied. E Result~ The purity of the samples after re-eryatallization was increased to 97.7%. The optimal conditions for phenol-sulfuric acid colorimetric method to determine the content of Jinggan^nyein were as follows： anhydrous glucose as control sample, 5% phenol, 1.0 ml phenol solution, 5.0 nfl sulfuric acid, stilly placed for20 min after reaction, 490 nm as detec- tion wavelength; the correlation coefficient of glucose standard curve （ R2 ） was 0. 993 9; the average recovery of standard addition was 99.72%, RSD was 0. 362 8%. ~ Conclusion] Re-cryatallization is a good way to refine Jinggan^nycin. Phenol-sulfuric acid colorimetric method can be used to determine the content of Jinggangmycin with simple operation, high accuracy and strong reliability, which can be widely applied in industrial production.
[ Objective ] In order to get the standard substance of Jinggangmycin used in quality control, Jinggangmycin was purified from its original pesticide and its content was determined, t Method] Mixed solvents were selected to recrystallize Jinggangmycin （content 62% ）. Phenol-sulfuric acid colofirnetric method was adopted to determine its content, and the effects of different determination conditions on the content results were also studied. E Result~ The purity of the samples after re-eryatallization was increased to 97.7%. The optimal conditions for phenol-sulfuric acid colorimetric method to determine the content of Jinggan^nyein were as follows： anhydrous glucose as control sample, 5% phenol, 1.0 ml phenol solution, 5.0 nfl sulfuric acid, stilly placed for20 min after reaction, 490 nm as detec- tion wavelength; the correlation coefficient of glucose standard curve （ R2 ） was 0. 993 9; the average recovery of standard addition was 99.72%, RSD was 0. 362 8%. ~ Conclusion] Re-cryatallization is a good way to refine Jinggan^nycin. Phenol-sulfuric acid colorimetric method can be used to determine the content of Jinggangmycin with simple operation, high accuracy and strong reliability, which can be widely applied in industrial production.
