摘要
目的 观察不同浓度As2 O3 对结肠癌Lovo、LS 174T细胞株生长的影响。方法 0 12 5～ 2 μmo1/L的As2 O3 与结肠癌细胞株Lovo、LS 174T共同孵育 ,观察不同浓度As2 O3 作用不同时间对结肠癌细胞的生长抑制作用、细胞形态学改变、DNA的变化。结果 1～ 2 μmol/LAs2 O3 作用的结肠癌细胞呈凋亡特征性改变 :细胞膜不发生破裂 ,细胞核出现核浓缩、核碎裂和凋亡小体形成 ;流式细胞仪检测在G1期前出现亚二倍体凋亡峰 ;细胞DNA抽提琼脂糖凝胶电泳呈凋亡特征性条带。结论 1～ 2 μmol/LAs2 O3 可诱导结肠癌细胞凋亡 ,肿瘤细胞凋亡与Fas、Fas
Objective To observe the effects of a wide range concentration of arsenic trioxide on the growth of colorectal cancer cell lines lovo and LS 174T.Methods Colorectal cancer cells were cultured with 0 125 2 μ mol/L arsenic trioxide for various duration.Cell activity morphologic changes and cell DNA distribution were studied.Results Colorectal cells cultured with 1～2 μ mol/L As 2O 3 presented apoptotic features:intact cell membrane,chromatin condenstation,nucleic fragmentation and apoptotic body formation.Flow cytometry analysis showed a sub G 1 cell peak.Agorose eletrophoresis showed marked DNA ladder.Conclusion 1～2 μ mol/L As 2O 3 can induce apoptosis of colorectal cancer cells and this apoptosis has some relation with the expression of Fas and Fas L.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第2期105-108,共4页
Cancer Research on Prevention and Treatment
