摘要
目的评价聚合酶键反应（PCR）检测肺地灌洗液人类巨细胞病毒（HCMV）对肺部HCMV感染的诊断价值。方法采用PCR技术检测保护性标本刷（PSB）洗涤液、肺泡灌洗液（BALF）静脉血白细胞HCMV－DNA保守区片段，扩增产物400bp。采用ELISA法检测血清HCMV－IgM。以PSBHCMVPCR检测结果为标准，比较BALF、静脉血白细胞HCMV－PCR、血清HCMV－IgGM的诊断价值。结果上述三个指标的敏感度、特异性、阳性预示值为：BALF－PCR；86．67％，96．67％，92．86％；静脉血白细胞PCR：26.67％，93．33％，66．67％；血清HCMV－IgM：80.00％，53．33％，46．15％。结论BALFHCMVPCR对肺部HCMV感染的诊断价值显著高于其他两种方法。
Objective To evaluate the diagnostic power of BALF HCMV PCR for HCMV infection of lung. Metheds Compared with venus white blood cell (WBC) and serum anti-HCMV IgM, the power of BALF HCMV DNA PCR was prospectinely investigated. HCMV DNA was measured by means of polymerse chain reaction,and the conservative segment of HCMV DNA was detected with the length of the amplified of HCMV DNA PCR 400bp. Anti-HCMV IgM was measured in ELISA. Results The sensitivity, specificity and predictive value were respectively (1)BALF HCMV DNA PCR: 86. 67%, 96. 67%, 92. 86%; (2) WBC HCMV DNA PCR: 26. 67%, 93. 33%, 66. 67%; (3) serum antiHCMV IgM: 80. 00%, 53. 33%, 46. 15%. Conclusion BALF HCMV PCR is obviously more valuable than the othel two methods in the diagnosis of HCMV infection of lung.
出处
《中国综合临床》
2000年第3期184-185,共2页
Clinical Medicine of China
