摘要
目的 :研究放射性核素 90 Y标记抗人成骨肉瘤单克隆抗体 (OSMc Ab)的条件及方法。方法 :采用 EDTA淋洗法从 90 Y发生器上制备游离 90 Y,并用 DTPA环酐法将 90 Y标记抗人成骨肉瘤单克隆抗体 (OSMc Ab)。标记后的单抗进行体外靶细胞结合实验以验证标记后单抗蛋白活性保持程度 ,以荷瘤裸鼠体内生物分布观察抗体蛋白特异性。 结果 :(1) EDTA淋洗法制备90 Y纯度高 ,其相对含量可达 99.997%。 (2 )单克隆抗体的 90 Y标记率达 90 %以上。 (3)本实验条件下使抗体蛋白活性保持82 .3%。 (4)经 OSMc Ab导向的 90 Y主要浓聚肿瘤组织。结论 :DTPA环酐法对 OSMc Ab行 90 Y标记操作简便实用 ,抗体蛋白活性及特异性保持良好。
Objective: To study DTPA coupled OSMcAb labeled with 90 Y. Methods: Carrier free 90 Y was obtained from its 90 Y/ 90 Sr generator and DTPA coupled OSMcAb was labeled with carrier free 90 Y. The protein activity of 90 Y DSMcAb was researched through the test of tumor cell OSMcAb in vitro . The specific of 90 Y DSMcAb was investigated with biodistribution in vivo . Results: (1) The 90 Y activity was higher while the contumination of 90 Sr was less than 0.003 1%; (2) The efficiency of 90 Y labeled antibodies was more than 90%; (3) The activity of labeled antibody maintained 82.3%. (4) 90 Y radioisotopes concentrated in tumor targeted by OsMcAb. Conclusion: OsMcAb can be successfully labeled with 90 Y by singgcyclic DTPA chelation.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第2期162-165,共4页
Academic Journal of Second Military Medical University
关键词
放射性核素标记
骨肉瘤
单克隆抗体
Y90
antibody, monoclonal
yttrium radioisotopes
radioisotopes labeling
osteosarcoma
