摘要
目的 :检测胎鼠及成年大鼠脑内神经干细胞特异性抗原神经巢蛋白 (nestin)的表达 ,判明啮齿类哺乳动物脑内神经干细胞存在的可能性及相应部位。方法 :显微手术下采取胎鼠及成年大鼠大脑皮质与皮质下脑组织 ,间接荧光法标记 nestin,以流式细胞术检测。结果 :胎鼠脑内普遍存在 nestin阳性细胞 ,成年大鼠脑室旁组织中也存在 nestin阳性细胞。结论 :胎鼠及成年大鼠的特定脑区存在神经干细胞。以流式细胞术检测神经干细胞特异性抗原 ,方法迅速、简便 ,结果可靠。
Objective: To determine the specific antigen of neural stem cell with flow cytometry. Methods: Fetal and adult rat brain were microsurgically obtained and fixed with monoclone antibody of nestin, the second antibdy of Ig PE were determined with flow cytometry. Results: There were nestin positive cells in both the brain tissues of fetal and adult rats. Conclusion: Determination of the nestin protein of neural stem cell with flow cytometry is a reliable method.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第2期117-119,共3页
Academic Journal of Second Military Medical University
基金
上海市科技发展基金!9941190 89
