摘要
对卷丹百合脱毒苗组培快繁技术体系进行研究。以带LSV病毒的卷丹百合珠芽为材料,通过36℃高温预处理10天,剥取0.2mm大小的茎尖在MS+2.5mg/L6-BA+1.5mg/LGA+0.5mg/LNAA+0.1g/L活性炭+0.6%琼脂+3%白糖的培养基中进行芽诱导培养,经过一次继代培养后,用RT-PCR检测LSV病毒,脱毒率达100%。将脱毒百合苗在MS+2.0mg/L6-BA+2.0mg/L2,4-D+0.1mg/LNAA培养基中诱导愈伤组织及丛生芽,诱导率达100%;在1/2MS+2.5mg/L6-BA+0.2mg/LNAA培养基中进行分化、增殖培养,增殖倍数达到4.31;采用MS+1.2mg/LNAA+0.5g/L活性炭培养基进行生根培养,产生的根系多而粗壮,移栽时最易成活。
In this work,Lilium lancifolium bulbils with LSV viruses were used as experiment material.Stripping 0.2 mm size of the shoot apex after 36℃ pretreatment 10 d,then culturing them in MS ＋ 2.5 mg/L 6-BA＋1.5 mg/L GA ＋0.5 mg/L NAA ＋0.1 g/L activated carbon ＋0.6% agar ＋3% sugar for bud induction,and detecting LSV virus using RT-PCR after a subculture,we found the virus-free rate to be 100%.The virus-free lily seedlings were put in MS ＋2.0 mg/L 6-BA ＋2.0 mg/L 2,4-D ＋0.1 mg/L NAA culture medium to induce callus and adventitious bud,induction rate 100%;in 1/2MS ＋2.5 mg/L 6-BA ＋0.2 mg/L NAA culture medium for differentiation and proliferation culture,proliferation multiples of Lilium lancifolium bulbils up to 4.31;in MS＋1.2 mg/L NAA＋0.5 g/L activated carbon culture medium for rooting,roots stout and branching vigorously,easy to survive after transplanting.
出处
《中国农学通报》
CSCD
2012年第31期201-205,共5页
Chinese Agricultural Science Bulletin
基金
湖南省蔬菜研究所青年创新基金"卷丹百合脱毒快繁技术研究"(hnsscyjs2011qncx06)
关键词
卷丹百合
茎尖脱毒
快繁技术
Lilium lancifolium Thunb.
shoot tip detoxification
rapid propagation
