摘要
目的:初步观察雄黄对MRL/lpr狼疮小鼠SLE动物模型抗双链DNA抗体及T细胞表面分子CD69、CD25表达及其生存时间的影响。方法:将MRL/lpr狼疮小鼠随机分为空白(蒸馏水)对照组、纳米雄黄低中高剂量组,每天灌胃一次,分别在不同时间点采集血清和称重;在实验结束后,取外周淋巴结分离T细胞并体外培养;放射免疫法测定血清抗ds-DNA抗体效价,流式细胞仪检测T细胞表面活化分子CD69、CD25表达水平;并计算各个观察点的小鼠死亡率。结果:雄黄治疗各组MRL/lpr小鼠生存时间比对照组明显延长,差异有统计学意义(P<0.05);雄黄低、中、高各组血清抗ds-DNA抗体总平均值显著低于对照组,其中高剂量与低剂量比较,差异也有统计学意义(P<0.05)。雄黄各剂量组T细胞表面的CD69和CD25表达水平较空白对照组有明显的降低,差异有统计学意义(P<0.05)。结论:雄黄可能通过抑制MRL/lpr小鼠T细胞表面的CD69和CD25表达以减少T细胞的活化,从而减少抗ds-DNA抗体的分泌,减轻小鼠自身免疫反应损伤,延缓SLE疾病发展进程。
Objective：To investigate the effects of Realgar on anti ds-DNA antibody and the expression of CD69,CD25 on MRL/lpr mouse T cell.Method：MRL/lprmice were divided into four groups：control group,Low dose realgar group,Medium dose realgar group,and High dose realgar group separation the peripheral lymph node during treatment,all mice were respectively collected serum and weighing in each time.In the end,T cells were cultured in vitro,which were separated from the peripheral lymph node in MRL/lpr mouse.Anti dsDNA antibody in serum were detected by radioimmunoassay,flow cytometry were used to detect the express of CD69 and CD25 by activated T cells in vitro in response to Concanavalin（Con A）.Result：The serum level of anti ds-DNA antibody in realgar groups were much lower than those in control group of MRL/lpr mice（P0.05）;Realgar treatment group MRL/LPR mice survival time was significantly longer than the control group（P0.05）;Realgar caused a dose-dependent suppression of CD69 and CD25 expression were significantly less than blank control group（P0.05）.Conclusion：Realgar can reduce T cell activation maybe by inhibiting expression of CD25and CD69 on T cell surface in MRL/lrp mouse,thus reducing anti ds-DNA antibody secretion,and reduce mouse damage by autoimmunnical response,delay SLE development process.
出处
《中国医学创新》
CAS
2012年第31期1-3,共3页
Medical Innovation of China
