摘要
以海南龙血树侧芽和顶芽为外植体,诱导愈伤组织进而分化成苗,同时利用愈伤组织诱导血竭。研究结果如下:①诱导愈伤组织与分化最适培养基为:1/2 MS+6-BA 2.0 mg/L+NNA 0.1 mg/L+CM 50 mL/L+糖30 g/L+卡拉胶8.6 g/L,诱导的愈伤组织结构致密、结实,分化的芽多,诱导达90﹪以上;②芽继代增殖最适培养基为:改良MS+6-BA 2.0 mg/L+NNA 0.1 mg/L+CM 50 mL/L+糖30 g/L+卡拉胶8.6 g/L,芽的长势好且整齐,增殖系数达5～6;③生根最适培养基为:不经过壮苗阶段直接从小苗进入生根阶段,1/2 MS+NNA 0.4 mg/L+GA 1.0 mg/L+糖30 g/L+卡拉胶8.6 g/L,根多且均匀,生根率达100%;④诱导出血竭后分离、提取龙血竭,经薄层层析和HPLC对照,发现愈伤诱导血竭与天然血竭的14种成分在HPLC峰形相似度较高。这一基于愈伤组织途径的组培技术兼顾了种苗生产和血竭生产的同步进行,为今后提供了高效、快捷、方便的规模化生产开辟了新思路。
Used the lateral and top buds of Dracaena cambodiana as the explants to induce callus tissue with that differentiated to seedling and induced dragon＇s blood at the same time. The results are as follows： The optimized culture medium for callus tissue induction and differentiation：①1/2MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L＋ CM 50 ml/L＋sugar 30 g/L＋arrageenan 8.6 g/L, buds of induction were more and neat, induction rate was more than 90%;②The Optimized culture medium for subculture proliferation of buds： modified MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L＋CM 50 ml/L＋sugar 30 g/L＋arrageenan 8.6 g/L, proliferation coefficient was up to 5-6; ③he Optimized culture medium for rooting： without strong seedling stage directly from seedling to rooting stages, 1/2MS＋NAA 0.4 mg/L＋GA 1.0 mg/L＋sugar 30 g/L＋arrageenan 8.6 g/L, rooting rate was up to 100%. ④After dragon＇s blood induction, separation and extraction, using thin-layer chromatography and HPLC-control, the study found that 14 ingredients had higher similarity between induced and natural dragonls blood in HPLC peak shape. The callus tissue culture technique gave consideration to the production of seedling and dragon＇s blood, and provided new ideas for an efficient, fast, convenient scale production in the future.
出处
《热带作物学报》
CSCD
北大核心
2012年第10期1824-1828,共5页
Chinese Journal of Tropical Crops
基金
林业公益性行业科研专项(No.201204604)
海南省重点科技计划项目(No.ZDXM 20120004)
农业公益性行业科研专项(No.201303117)
关键词
海南龙血树
愈伤组织诱导
血竭
Dracaena cambodiana
Callus tissue induction
Dragon＇s blood
