摘要
以海南龙血树的顶芽和侧芽作为外植体,把其接种于MS+BA1mg/L+NAA0.1mg/L+PVP100mg/L+蔗糖30g/L培养基上培养40￣50d可诱导其腋芽萌发,再把萌发后所形成的新芽切割下来接种于MS+BA2mg/L+KT0.5mg/L+蔗糖30g/L的培养基上培养25￣30d可诱导形成丛生芽,丛生芽在继代培养过程中每25￣30d可增殖3￣5倍。把丛生芽分割成单株并接种于MS+BA3mg/L+GA31mg/L+蔗糖30g/L培养基上培养4周后使其壮苗后再接种于MS+NAA0.5￣1.0mg/L+蔗糖30g/L培养基上培养4周可诱导小芽形成完整的根系,小植株移栽成活率可达98%。该体系的建立为海南龙血树的工厂化育苗奠定了坚实的基础。
The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40-50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25-30 d, the clustered buds formed. The proliferation rate of the clustered buds was 300 %-500 % per month. The clustered buds were cultured on MS medium containing 3 mg/L BA, 1 mg/L GA3 and 30 g/L sucrose for about 30 d to make the shoots stronger. These shoots were transferred onto MS medium containing 0.5-1.0 mg/L NAA and 30 g/L sucrose for 25-30 d for inducing roots. About 98 % of plantlets survived after they were transferred onto soil. This cultural system will be a good foundation for commercial proliferation of D. cambodiana.
出处
《热带作物学报》
CSCD
2005年第4期20-23,共4页
Chinese Journal of Tropical Crops
关键词
海南龙血树
组织培养
快速繁殖
Dracaenacambodiana Pierre ex Gagnep. tissue culture rapid propagation
