摘要
研究了酸性条件下染色剂苋菜红-蛋白质体系的共振瑞利散射光谱。在pH=3.80的Clark-Lubs缓冲介质中,苋菜红与蛋白质通过分子间作用力形成复合物,使最大波长为523nm的共振光散射光谱得到加强,这种增强的共振光散射用于鸡蛋清试样中总蛋白的测定,其线性响应范围为1.5～5.0mg/L,检测限为209μg/L。该方法的稳定性及选择性良好,适用于微量蛋白质分析。
Resonance light scattering （RLS） spectrum of Amaranth -Protein system was studied in this paper. In Clark-Lubs buffer medium of pH 3.80, the Amaranth-Protein composites formed via intermolecular forces, which enhancing the resonance light scattering at maximum wavelength of about 523 nm. Based on the enhancement of RLS, a novel method for the determination of proteins was developed. The line arrange for the determination of protein is 1.5 to 5.0 mg/L, limit of detection is 209μg/L. The method is stability and selectivity, which can be used to determine trace protein.
出处
《皖西学院学报》
2012年第5期106-109,共4页
Journal of West Anhui University
