摘要
实验室条件下,研究了3种贝类（太平洋牡蛎、紫贻贝、菲律宾蛤仔）不同浓度组织匀浆液及5种单一L型氨基酸（甘氨酸、谷氨酸、酪氨酸、天冬氨酸、赖氨酸）等化学刺激物对多棘海盘车（Asterias amurensis）摄食行为及呼吸代谢的影响。将多棘海盘车对刺激物的行为反应分为正趋向、负趋向和无反应3类,趋向反应由简单到复杂分为4种反应类型,通过测定耗氧率来表示呼吸代谢水平。结果显示,3种贝类组织匀浆液和5种氨基酸（浓度范围10 10~10 4mol/L,酪氨酸除外）均引起多棘海盘车的正趋向反应,且多棘海盘车产生反应的数量比例随刺激物浓度的升高而增加,反应类型也由简单逐渐趋于复杂。同种氨基酸在低浓度下引起多棘海盘车正趋向反应,但超过一定浓度（10 3~10 2mol/L）后会引起负趋向反应（实验浓度范围内谷氨酸组未出现）。不同种类刺激物对多棘海盘车摄食行为反应的影响不同,谷氨酸、甘氨酸以及菲律宾蛤仔组织匀浆液对多棘海盘车诱引效果最好。多棘海盘车的耗氧率随刺激物浓度的增加而逐渐升高。在贝类组织匀浆液中多棘海盘车的耗氧率均高于对照组;甘氨酸、谷氨酸、天冬氨酸各处理组中,只有在较高浓度下耗氧率才显著高于对照组,酪氨酸各组之间无显著差异。研究旨在为贝类底播增养殖和人工鱼礁区敌害生物的防除提供参考,同时为多棘海盘车觅食行为机制的研究提供数据支持。
Experiments were carried out to investigate the behavioral and metabolic responses of the North Pacific sea star（Asterias amurensis） to different concentrations of food signals（five single amino acid and three bivalve tissue homogenate）.To gain greater knowledge into the foraging behavior of A.amurensis,its behavior was di-vided into four components.Stimulus concentration increase elicited a rise in both the percentage of reacting ani-mals and in the reaction intensity at the amino acid concentrations of 10？4–10？10 mol？L？1 except for tyrosine.At a low stimulus concentration,the reaction of A.amurensis consisted of tube foot waving,and only high concentra-tions initiated a complicated sequence of several types of behavior.However,fright reactions were observed at high concentrations（10？3–10？2 mol？L？1） of amino acid except for glutamic acid.Differences were found among the behavior response by A.amurensis exposed to different types of stimuli.Glutamic acid,glycine and clam Rudi-tapes philippinarum homogenates were the strongest attractant for the studied sea stars.For the eight tested stimuli,a dependency between stimulus concentration and the oxygen consumption rate（OCR） was observed,with the increase in stimulus strength causing the increase in the metabolic rate.For the tissue homogenates groups,the OCR was significantly higher than that in the control.However,statistically significant differences from the con-trol were only found in sea stars exposed to high concentrations of glycine,glutamic acid and aspartic acid.No significant differences were observed among different concentrations of tyrosine.
出处
《中国水产科学》
CAS
CSCD
北大核心
2012年第6期978-988,共11页
Journal of Fishery Sciences of China
基金
农业部公益性行业(农业)科研专项(201003068)
关键词
多棘海盘车
摄食行为
化学感觉
氨基酸
组织匀浆液
Asterias amurensis
foraging behavior
chemoreception
amino acid
tissue homogenates
