摘要
目的探讨氟离子导入后乳牙牙釉质表层氟含量的变化和釉质磷灰石晶胞结构的变化。方法 40颗下颌乳中切牙,随机编号1～40号,对半切开,选取1～20号标本的一半为导入组,20～40号标本的一半为浸泡组,选取1～40号标本另一半偶数组作为对照组。导入组以2%NaF溶液为导入液进行氟离子导入处理,浸泡组以2%NaF溶液进行浸泡,对照组以生理盐水进行浸泡,每天20 min,持续10 d,处理完成后标本用环氧树脂包埋。应用电子探针测量仪检测釉质表面下100μm分析微区内釉质氟含量,并用X线衍射仪对釉质表面下100μm分析微区进行X线衍射检测,观察釉质晶胞结构的变化。结果釉质表面下100μm内氟含量,导入组高于浸泡组,浸泡组又高于对照组(P<0.01)。X线衍射图谱显示,3组乳牙标本的X线衍射峰形明显不同于羟基磷灰石,但相互间基本相似。3组乳牙标本晶体的a轴均大于羟基磷灰石,c轴均小于羟基磷灰石(P<0.01),而3组乳牙标本间晶胞轴长差异无统计学意义(P>0.01)。结论氟离子导入可以有效提高乳牙釉质表层的氟含量,乳牙釉质氟离子的导入并没有引起釉质晶胞轴长的改变。
Objective To investigate the changes of fluoride contents and apatite crystal structure under the surface of enamel in deciduous teeth after fluoride iontophoresis. Methods Forty lower central incisors were labeled and cut in half. The half part of 20 specimens were selected as iontophoresis group in which fluoride iontophoresis was conducted in 2% NaF solution, the half of other 20 specimens were selected as immersion group which were immersed in 2 % NaF solution. The half of 10 specimens in both groups were selected as controls which were immersed in saline. The process was carried out for 20 min a day and continued for 10 d. The contents of fluoride were measured byElectron Probe Micro-Analysis （EPMA）, and the X-ray Diffraction （XRD）spectrum was obtained in 100 μm zone under the surface of enamel. Results The fluoride contents in 100 μm zone under the surface of enamel of iontophoresis group was higher than those of immersion group, while the fluoride contents of immersion group was higher than those of control group. The XRD spectrum showed the patterns of three deciduous teeth groups were different to that of hydroxyapatite distinctly, while were similar to each other. The axis A of the crystal of three deciduous teeth groups was longer than that of hydroxyapatite, while the axis C was shorter than that of hydroxyapatite. There was no difference in the length of axis A and C among three deciduous teeth groups. Conclusion The fluoride contents under the enamel surface can be increased effectively by fluoride iontophoresis. The cell-edge length of enamel crystal is not changed by fluoride iontophoresis.
出处
《同济大学学报（医学版）》
CAS
2012年第5期33-36,共4页
Journal of Tongji University(Medical Science)
基金
上海市卫生局青年科研基金(2007Y10)
关键词
乳牙
釉质
氟
离子导入
deciduous teeth
enamel
fluoride
iontophoresis
