摘要
首次从中草药龙葵 Solanum nigrum L.的稀碱提取液中分离得到 2种多糖 SNL- 3,SNL- 4。分别经琼脂糖凝胶电泳、凝胶柱层析法分析 ,证实均为单一组分。 SNL - 3,SNL - 4经酸水解、Sm ith降解、红外等确知 ,SNL- 3由 D-木糖 (75 .7% )、D-甘露糖 (9.2 % )、D-葡萄糖 (4.9% )和 D-半乳糖 (10 .2 % )组成 ,分子量为 2 370 0 ;SNL - 4由 D-木糖 (89.4% )、D -甘露糖 (1.7% )和 D-半乳糖 (8.9% )组成 ,分子量为 4770 0。 SNL - 3和SNL- 4均以 1。
Water soluble polysaccharides SNL 3, SNL 4 were isolated from Solanum nigrum L.. Each of them was shown to be a single homogeneous component by agarose gel electrophoresis and Sephadex column chromatography. Their molecular weights were respectively estimated to be 23 700 and 47 700 . Acid hydrolysis, periodate oxidation, Smith degradation and IR spectroscopic analysis showed that SNL 3 contained D xylose (75 7%), D mannose (9 2%), D glucose (4 9%) and D galactose (10 2%); SNL 4 contained D xylose (89 4%), D mannose (1 7%) and D galactose (8 9%). Both of them were mainly composed of Xy Ⅰ residues via β (1→3) glucosidic linkage.
出处
《中草药》
CAS
CSCD
北大核心
2000年第3期162-164,共3页
Chinese Traditional and Herbal Drugs
关键词
龙葵
龙葵多糖
分离
纯化
鉴定
中药
Solanum nigrum L. polysaccharides from Solanum nigrum L. immunologic competence