摘要
目的 分离、纯化和鉴定壮阳中药肉苁蓉 Cistanche deserticola中的多糖成分。方法 经脱脂、沸水抽提、乙醇沉淀、Sevag法脱蛋白得到的粗多糖 ,再经 DEAE- Sephadex A- 5 0离子交换层析和 Sephacryl S- 2 0 0凝胶柱层析得到纯净的肉苁蓉多糖 (CDPS) ,用红外光谱和紫外光谱分析鉴定该多糖。结果 红外光谱分析具有典型的多糖特征吸收峰 ,紫外光谱分析未见核酸和蛋白质的特征吸收峰。结论 本实验所提取的肉苁蓉多糖为单一、纯净的多糖。
Object The polysaccharide (CDPS) was isolated, purified and identified from Cistanche deserticola Y. C. Ma, a Chinese materia medica. Methods The polysaccharide was extracted with hot water and precipitated by alcohol. Protein in the precipitates was removed by Sevag method. The products were further purified with column chromatography on DEAE-Sephadex A-50 and Sephacryl S-200. The CDPS was idendified by IR spectrum and UV (200-400 nm) scanning spectrum. Results IR spectrum indicates that there are typical characteristic absorption peaks of polysaccharides. UV scanning spectrum shows that there are no absorption peaks of protein and nucleic acid at point 280 nm and 260 nm. Conclusion The CDPS was identified as homogeneous one.
出处
《中草药》
CAS
CSCD
北大核心
2002年第12期1057-1059,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目 (No.3 93 0 0 174)
关键词
分离
纯化
鉴定
肉苁蓉多糖
柱层析
光谱分析
polysaccharide of Cistanche deserticola Y. C. Ma (CDPS)
column chromatography
spectrum analysis
