摘要
目的 研究 β 环糊精 14硫酸酯 (β CD 14S)抑制肿瘤新生血管的作用机制。 方法 采用3 H 胸腺嘧啶核苷 (3 H TdR)掺入法 ,检测 β CD 14S单独及与氢化可的松 (HC)联合应用 (以各种药物加药浓度不同分别分为 5个组 )对血管内皮细胞、正常肝细胞系 (L0 2 )细胞 ,人肝癌 772 1细胞和肝癌Waker 2 5 6细胞增殖的影响。结果 各种不同浓度的 β CD 14S、HC以及联合用药对内皮细胞生长率有明显的抑制 ;与L0 2 ,772 1,Waker 2 5 6细胞相比较 ,差异均有显著性 (P <0 .0 0 1)。联合用药较单独用药对内皮细胞生长率的抑制更加明显 (P <0 .0 0 1)。结论 β CD 14S具有抑制新生血管形成和药物靶向载体的双重功能。
Objective To study the mechanims of β cyclodextrin(CD) tetradecasulfate (14S) inhibiting the solid tumor angiogenesis. Methods Proliferation rates of microvascular endothelial cells (MECs), human hepatocyte(L02 hepatocyte line), 7721 and waker 256 hepatoma line were measured by using 3H TdR method after treated with β CD 14S alone or combined with hydrocortison. Results The proliferation of MECs can be inhibited by β CD 14S, hydrocortison alone or combined more effectively than that of other three cell lines (P<0.01). In MECs,the inhibition of combination was more serious than that of either β CD 14S or hydrocortison (P<0.01). Conclusion β CD 14S might play a dual function role as an antiangiogenesis agent and a drug carrier in tumor therapies, including treatment of liver cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第2期165-166,共2页
Chinese Journal of Experimental Surgery
关键词
新生血管形成
肝细胞癌
β-CD-14S
Cyclodextrin tetradecasulfate
Angiogenesis
Endothelial cell
Carcinoma,hepatocellular
