摘要
目的探讨p21基因转染人肝癌SMMC-7721细胞（7721细胞）对化疗药物的敏感性，深入研究p21基因对肝癌细胞的治疗作用。方法应用磷酸钙介导p21基因转染人肝癌7721细胞；通过G418筛选稳定表达细胞株并扩大培养；采用MTT法检测肿瘤细胞增殖速度；流式细胞术检测细胞周期变化。结果经过3～4w G418筛选得到稳定表达的细胞株，p21基因稳定转染并联合化疗药物应用可明显抑制7721细胞的体外增殖，细胞周期明显改变，细胞从G1期到G2期发生阻滞。结论提示转染外源p21基因联合化疗药物可使7721细胞周期阻滞于G1期，并可抑制肿瘤细胞增殖。
Objective To explore the sensitivity of the human liver cancer cells transfected with p21 gene to chemotherapeutic drugs, and further understand the mechanism of the tumor suppressor gene p21. Methods The p21 gene induced by calcium phosphate was transfected into human liver cancer cell line （7721）. The stable expression cells were selected by G418. The proliferation of tumor cells was examined by MTT assay, and the changes of cell cycle progression were observed by flow cytometry （FCM）. Results The stable expression cells were obtained after selected by G418 for 3 -4 w. Proliferation of 7721 was inhibited significantly by stable p21 gene transfected and chemotherapeutic drugs in vitro. The progression of cell cycle was arrested from G1 to G2 phase. Conclusions The growth of 7721 cell could be arrested at G1 phase, and it could be significantly suppressed by exogenous p21 gene transfected and chemotherapeutic drugs.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第9期1221-1223,共3页
Chinese Journal of Gerontology
关键词
P21基因
肝癌细胞株
MTT法
基因转染
p21 gene
Hepatocellular carcinoma cell line
MTr assay
Gene transfectio n
