摘要
目的 探讨大肠癌患者骨髓中微转移基因检测的意义。 方法 应用聚合酶链式反应 单链构象多态性分析 (PCR SSCP) /银染技术 ,检测 5 1例大肠癌患者手术前、后不同时间骨髓中p5 3和K ras基因的突变状况。 结果 5 1例患者术前骨髓中基因突变阳性者 19例 ,阳性率 37 2 5 %。阳性检出与Duke′s分期、淋巴转移显著相关。术后经 2～ 3个疗程的化疗后 ,19例基因突变阳性的患者中11例转阴。 结论 大肠癌患者骨髓转移癌细胞的基因检测 ,有助于大肠癌血路转移的早期诊断 。
Objective To detect the gene of micrometastasis in bone marrow of patients with colorectal cancer. Methods PCR SSCP/silver stain technique was used to find out the metastatic cancer cells in 51 bone marrow samples in different time. Results The total positive rate of mutations of p53 and K ras in bone marrow was 37 25% before operation. The incidence of mutations was obviously correlated with Duke′s stage and lymphatic metastasis. After postoperative chemotherapy mutation in 11 patients turned to be negative. Conclusions Detection of micrometastasis in bone marrow may contribute to early diagnosis of blood stream metastasis of colorectal cancer.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2000年第3期196-197,共2页
Chinese Journal of Surgery
关键词
大肠肿瘤
肿瘤转移
骨髓微转移
基因突变
Colorectal neoplasm
Neoplasm metastasis
Bone marrow
Mutation
Gene
