期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

白血病细胞多巴胺受体基因甲基化研究 被引量：1

Study on aberrant DNA methylation of dopamine D4 receptor gene in acute myeloid leukemia
暂未订购
导出
摘要 目的 :探讨多巴胺受体 ( DRD4 )基因第一外显子中 Not 酶切位点甲基化与急性非淋巴细胞白血病( AML)的关系。方法 :应用 Southern印记杂交及 DNA序列分析技术对 AML 患者骨髓标本进行分析。结果 :在 2 7例白血病患者骨髓标本中 16例 DRD4基因甲基化 ( 5 9% ) ,其中初治化疗敏感患者 DRD4基因甲基化发生率为5 0 % ( 9/18) ,复发及难治患者甲基化发生率为 78% ( 7/9) ,而 9例正常人骨髓标本 DRD4基因均无甲基化。结论 :DRD4基因甲基化在白血病患者发生率明显高于正常人 ( P<0 .0 5 ) ,而在复发及难治患者其发生率略高于初治化疗敏感患者 ,DRD4基因甲基化可能与白血病的发生。 Objective:To investigate aberrant DNA methylation of Not I site on exon I of dopamine D4 receptor (DRD4) gene in acute myeloid leukemia (AML).Methods:Southern Blot and DNA sequencing technique were performed on DNA from AML bone marrow samples. Results:Sixteen samples from 27 AML patients (59%) showed DNA methylation of DRD4 gene. Nine samples from 18 diagnostic chemosensitive patients (50%) and 7 samples from 9 refractory and relapse AML patients (78%) showed DNA methylation. But 9 normal bone marrow samples did not show any DNA methylation on DRD4 gene. Conclusion:The incidence of DRD4 gene methylation in AML was significantly higher than that in normal bone marrow ( P <0 05). There is potential importance of DRD4 gene methylation in initiation and development of AML.
作者 于力 王全顺 顾群 史子江 靳海杰
机构地区 解放军总医院血液科
出处 《军医进修学院学报》 CAS 2000年第1期62-64,共3页 Academic Journal of Pla Postgraduate Medical School
基金 "九五"军队科研基金!项目 ( 98M14 1)
关键词 急性白血病 多巴胺受体 DNA甲基化 AML DRD4 gene DNA methylation
分类号 R733.710.2 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1[1]Costello JF, Plass C, Arap W et al. Cyclin dependent kinase 6 (CDK6) amplification in human glioblastomas identified using two-dimensional separation of genomic DNA [J]. Cancer Res, 1997,57(7):1250-1254.
  • 2[2]Sambrook J, Fritsch EF, and Maniatis T. Molecular Cloning: A laboratory manual [C]. 2nd Ed. Cold Spring Harbor Lab Press: Plainvies NY, 1989.
  • 3[3]Chio CL, Drong RL, Riley RF et al. D4 dopamine receptor-mediated signaling events determined in transfected chinese hamster ovary cells [J]. J Biol Chem, 1994,269(16):11813-11819.
  • 4[4]Furuya KN, Thottassery JV, Schuetz EG et al. Bromocriptine transcriptionally activates the multidrug resistance gene (pgp/mdr1b) by a novel pathway [J]. J Biol Chem, 1997,272(17):11518-11525.
  • 5[5]Kamakura S, Iwaki A, Matrumoto M et al. Cloning and characterization of the 5′-flanking region of the human dopamine D4 receptor gene. Biochem Biophys Res Commun [J], 1997,235(2):321-326.
  • 6[6]De Bustros A, Nelkin BD, Silverman A et al. The short arm of chromosome 11 is a “hot Spot” for hypermethylation in human neoplasia [J]. Proc Nat1 Acad Sci USA, 1988,85(15):5693-5697.
  • 7[7]Baylin SB, Herman JG, Graff JR et al. Alterations in DNA methylation: A fundamental aspect of neoplasia [J]. Adv Cancer Res, 1998,72:141-196.
  • 8[8]Herman JG, Civin CI, Issa JP et al. Distinct patterns of inactivation of p15INK4Band p16INK4A characterize the major types of hematological maliganancies [J]. Cancer Res, 1997,57(1):837-841.
  • 9[9]Issa JP, Zehnbauer BA, Civin CI et al. The estrogen receptor CpG island is methylated in most hematopoietic neoplasms [J]. Cancer Res, 1996,56(5):973-977.
  • 10[10]Momparler RL, Cote S, Eliopoulos N et al. Pharmacological approach for optimization of the dose schedule of 5-aza-2′-deoxycytidine (Decitabine) for the therapy of leukemia [J].Leukemia,1997,11(2):175-180.

同被引文献12

  • 1Cody DT,Huang Y. Differential DNA methylation of the p16 INK4A/CDKA2A promoter in human oral cancer cells and normal human oral kerarinocytes[ J] .Oral Oncol, 1999,35(5) :516-522.
  • 2Liu M, Taketani T, Li R, et al. Loss of p73 gene expression in lymphoid cell lines is associated with hypermethylation [ J ]. Leuk Res, 2001,25(6) :441-447.
  • 3Toyooka KO, Toyooka S, Virmani AK, et al. Loss of expression and methylation of the CDH13 (H-cadherin) gene in breast and lung carcinomas[J]. Cancer Res,2001,61 ( 11 ) :4556-4560.
  • 4Lyon MF. Imprinting and X-chromesome inactivation [ J ]. Result Probl Cell Differ, 1999,25(8) :73-90.
  • 5Peter WL, Rudolf J. The role of DNA methylation in cancer genetics and epigenetics[J]. Aunn Res Genet, 1996,30(9):441.
  • 6Bender CM, Zingg JM, Jones PA. DNA methylation in bladder cancer[J]. Pharm Aceutical Res, 1998,15(2) : 175.
  • 7Ahujia N. Aging and DNA methylation in colorectal mucosa and cancer[J]. Cancer Res, 1997,58(23) :3370-3374.
  • 8Wheeler JM, Beck NE, Kim HC, et al. Mechanisms of inactivation of mismatch repair genes in human colorectal cancer cell lines: the predominant role of Hmlh1 [ J] .Proc Natl Acad Sci USA, 1999,96(18) :10296-10301.
  • 9Ng HH, Bird A. DNA methylation and chromatin modification[ J]. Curt Opin Genet Dev, 1999,9(2) : 158-163.
  • 10Costello JF, Fruhwald MC. Aberrant CpG-island methylation has nonrandom and tumour-type-speeitic patterns [ J ]. Nat Genet, 2000,24(2) :132-138.

引证文献1

二级引证文献1

军医进修学院学报
2000年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部