摘要
目的:建立中药芪胶膏的质量标准。方法:采用TCL法对制剂中黄芪和三七进行定性鉴别,采用HPLC-ELSD法测定黄芪甲苷的含量。结果:TCL鉴别色谱特征斑点明显。黄芪甲苷进样量在1.90464～13.9674μg范围内时,质量与峰面积积分值呈良好的线性关系(r=0.9991,n=6),平均加样回收率为98.52%,RSD=3.7%(n=6)。结论:所建立的TLC和HPLC-ELSD法专属性强,重复性好,可用于芪胶膏的质量控制。
OBJECTIVE To establish the quality standard of Qijiao Gao.METHODS The qualitative identifications of Astragalus mongholicus and Pseudo-ginseng were carried out by TLC,and the content of astragaloside was determined by HPLC.RESULTS The developed TLC spots were quite clear.The linearity was from 1.904 64 to 13.967 4 μg for astragaloside by HPLC,and the average recovery was 98.52% for astragaloside with RSD of 3.7%（n=6）.CONCLUSION The methods of established TLC and HPLC are reliable,accurate,specific,reproducible,and suitable for the quality control of Qijiao Gao.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2012年第21期1731-1734,共4页
Chinese Journal of Hospital Pharmacy
基金
四川省中医药管理局科技立项课题(编号:2010-76)
关键词
芪胶膏
黄芪甲苷
黄芪
三七
质量控制
Qijiao Gao
astragaloside
Astragalus mongholicus
Pseudo-ginseng
quality control.
