摘要
通过大孔吸附树脂、葡聚糖凝胶、薄层制备硅胶板(TLC)及制备高效液相色谱(HPLC)从哈茨木霉菌Trichoderma harzianum发酵液中分离、纯化出4种由20个氨基酸组成的线性peptaibols抗菌肽FD1、FD2、FD3、FD4。经ESI-IT-MS串联质谱鉴定,其分别为抗菌肽Alamethicin F-50、Atroviridin B、Alamethicin II和Atroviridin C。菌丝生长及孢子萌发实验表明其对水稻纹枯病菌Rhizoctonia solani AG4、黄瓜立枯病菌R.solani、黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinum、西瓜枯萎病菌F.oxysporum f.sp.niveum、水稻稻瘟病菌Magnaporthe oryzae具有较好的抑制作用,同时发现木霉素Trichodermin与peptaibols抗菌肽Alamethicin F-50联合作用时对菌丝生长具有协同抑制作用。
Four microheterogenous peptaibol antibiotics containing 20 amino acids were isolated from fermentation broth of Trichoderma harzianum and purified by polystyrene resin, sephadex, preparative thin-layer chromatography （TLC） and preparative high-performance liquid chromatography （HPLC）. They were identified as Alamethicin F-50, Atroviridin B, Alamethicin II and Atroviridin C with ESI-IT-MS2. Mycelial growth and spore germination experiments showed that the 4 compounds have strong inhibiting effects against Thanatephorus cucumeris, Rhizoctonia solani, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. niveum, Magnaporthe oryzae, and Alamethicin F-50 exerted synergistic antibiotic effect when applied with trichodermin on the tested plant pathogens.
出处
《中国生物防治学报》
CSCD
北大核心
2012年第4期528-536,共9页
Chinese Journal of Biological Control
基金
浙江省科技厅重大科技专项(2008C02007-2)
关键词
哈茨木霉菌
抗菌肽
抑菌活性
Trichoderma harzianum
peptaibol antibiotics
antibiotic activity
