摘要
目的:建立白及人工种子的制作方法,并考察相关影响因素,为白及的繁育提供一条新途径。方法:以白及种子为材料,在1/2MS培养液中萌发后作为人工种子的包埋材料,研究了人工胚乳组分、人工种皮基质、贮藏条件、萌发基质等对人工种子萌发率和成苗率的影响。结果:以4.0%海藻酸钠+0.2 mol·L-1CaCl2+0.4 mg·L-1青霉素+0.3%多菌灵粉剂+0.2%苯甲酸钠为人工种皮基质,MS+1.0 mg·L-1NAA+2.0 mg·L-1KT作为人工胚乳成分制成的人工种子,其生长速度和成苗率最高。人工种子在4℃条件下贮藏较长时间还可保持较高的活力。结论:通过研究确立了白及人工种子制作基本技术,但其在有菌环境中的播种技术还有待进一步研究改进。
Objective： To establish a new manufacturing method for Bletilla striata synthetic seeds, and provided a new way for rapid propagation of B. striata, the correlated influential factors were studied. Method： The synthetic seeds were manufactured by taking seeds of B. striata as materials which were beforehand germinated in 1/2 MS medium for 10 days, and the influential factors such as artificial endosperm components, episperm substances, storage conditions and germination groundmass impact on the germination rate and seedling rate of the synthetic seeds were evaluated. Result ： Compound 4.0% sodium alginate ＋ 0.2 mol · L^-1CaC12 ＋ 0.4 mg · L^- 1 penicillin ＋0.3% carbendazim powder ＋ 0.2% sodium benzoate served as the best episperm substances while MS ＋ 1.0 mg· L^- 1 NAA ＋ 2.0 mg · L^- 1 KT as the best endosperm components, in which, high germination rate and seedling rate were obtained. The syn- thetic seeds storing in the 4 ℃ for a long time was able to have still high vitality. Conclusion： The B. striata synthetic seeds manufactur- ing system was established successfully, while efforts should be taken to improve the sowing technique of the synthetic seeds in non-sterile comiitions.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第22期3386-3390,共5页
China Journal of Chinese Materia Medica
基金
浙江省公益性技术应用研究计划项目(2011C22047)
浙江省大学生科技创新活动计划项目(2011R410019)
关键词
白及
人工种子
快繁
贮藏
Bletillae striata
synthetic seeds
rapid propagate
storage
