摘要
采用SDS-PAGE测定了82个副猪嗜血杆菌分离株细胞外膜蛋白(OMP),比较不同临床背景分离株的OMP表型差异,分析了OMP与毒力菌株的相关性。结果表明,外膜蛋白分为3种类型:PAGE I、PAGE II和PAGEⅢ型。约34%患病猪分离株属于PAGE I型,只有约9%健康猪分离株属于PAGE I型,说明36 000～40 000蛋白与菌株的毒力相关。
The out membrane protein （OMP） in 82 isolations of Haemophilus Parasuis were deter- mined by SDS-PAGE. We compared the diversity of the OMP phenotype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen. The results showed that isolates are divided into 3 major PAGE type groups as differece of Rf. PAGE Ⅰ is char- acterized betwee 36 000 and 40 000 OMP,and included about 34% isolates recovered from disease pigs and 9% isolates recovered from healthy pigs; PAGE Ⅱ is characterized by absence of the 36 000-40 000 proteins. It is shown that the 36 000-40 000 OMP could be related to virulence.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第11期1656-1661,1668,共7页
Chinese Journal of Veterinary Science
基金
香港霍英东铭源基金(HK314-14591)
广东省农业攻关项目(2004B20201019)
韶关市科技创新项目(2010140473)
关键词
副猪嗜血杆菌
外膜蛋白
毒力菌株
Haemophilus parasuis
out membrae protein
virulen pathogen
