摘要
为了揭示副猪嗜血杆菌外膜蛋白与毒力的关系,采用SDS-PAGE测定了82个副猪嗜血杆菌分离株细胞外膜蛋白(OMP),比较了不同临床背景分离株的OMP表型差异,根据外膜蛋白的电泳迁移率Rf值和蛋白含量对OMP与毒力菌株的相关性和PAGE分型进行了聚类分析。图谱表型分析结果表明,82个分离株分成以分子量36～40KD为特征的Ⅰ型和以42～45KD为特征的Ⅱ型2种类型,约34%患病猪分离株属于PAGEⅠ型,只有约9%健康猪分离株属于PAGEⅠ型。Rf值聚类分析结果显示外膜蛋白分为3种类型,PAGEⅠ、PAGEⅡ和PAGEⅢ型。图谱蛋白含量聚类分析显示5个PAGE型。结果提示,36～40KD蛋白与菌株的毒力相关。
To explose relatioship between out menbrane protein and virulence of Haemophilus Parasuis.The out membrane protein（OMP） in 82 isolations of Haemophilus Parasuis were determined by SDS-PAGE.It compared the diversity of the OMP phenotype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen,and analyzed relationship between the OMP and virulen isolates by cluster analysis base on Rf of electrophoresis and contents of OMP.The results of analysis of OMP phenotye showed that isolates were divided into two major PAGE type groups as differece of Rf.PAGE I was characterized between 36 KD and 40 KD OMP and PAGEⅡwas characterized 42-47 KD OMP,and included about 34% isolates recovered from disease pigs and 9% isolates recovered from healthy pigs;PAGE II was characterized by absence of the 36~40 KD proteins.The result of cluster as Rf indicated that isolates were divided three types：PAGEⅠ,PAGEⅡand PAGEⅢ.Analysis of cluster as contents of OMP obtained five classsifications of isolates.It is prompted that the 36~40 KD OMP was correlation likely with virulence.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2011年第6期579-583,588,共6页
Journal of Nanchang University(Natural Science)
基金
香港霍英东铭源基金资助项目(HK314-14591)
广东省农业攻关基金资助项目(2004B20201019)
韶关市科技创新基金资助项目(2010140473)
