摘要
采用小鼠卵母细胞体外培养、体外受精的方法研究不同产地 3种香烟的烟雾水溶物对小鼠卵母细胞成熟和受精能力的影响。结果显示 3种香烟烟雾水溶物对卵母细胞生发泡破裂都没有影响 ,但可以显著地抑制卵母细胞第一极体的释放、降低卵母细胞的体外受精率和体外存活率。结果提示 ,香烟烟雾中影响生殖作用的主要成分是其中的水溶性成分 ,它们可以破坏卵母细胞的成熟、降低卵细胞的受精能力 ,对生殖细胞和生育能力有一定的毒性作用 ;不同产地、不同品牌香烟烟雾水溶物毒性作用相似。
In this Paper,the effect of three kinds of water-soluble contents of cigarette smoke(WSCS) from different places of production on in vitro meiotic maturation and in vitro fertilization(IVF)of mouse oocyte was studied by culture of mouse oocyte and IVF.The results showed that WSCS could inhibit the extruding of the first polar body and reduce the rate of IVF.It could also affect the quality and the viability of mouse oocyte with little impact on the germinal vesicle breakdown(GVBD).The results indicated that the major components of cigarette smoking were water-soluble contents which may be hazardous to the meiotic maturation of mouse oocyte and reduce the fertile capacity and result in toxicity to women's oocyte and feartility function.the results also showed that the toxicity effects of different brands from differen places of production might be similarity.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第5期406-408,共3页
Chinese Journal of Public Health
关键词
香烟烟雾水溶物
卵母细胞
生发泡破裂
体外受精
Water-soluble contents of cigarette smoke(WSCS) Oocyte Germinal vesicle breakdown(GVBD) the First polar body in Vitro fertilization(IVF)
