摘要
采用稀释平板涂布法对29份根际土样进行分离,共获得239株放线菌,采用平板对峙培养法筛选得到48株对烟草黑胫病菌有拮抗作用的菌株,占筛选菌株的20.1%,根据形态和培养特征,48株拮抗放线菌被鉴定为链霉菌属(Streptomyces)和诺卡氏菌属(Nocardia),以链霉菌属为主,占拮抗菌的97.92%。对拮抗作用较好的4株放线菌进行了发酵液拮抗活性的测定,结果表明,4株放线菌平均抑制率为59.80%,其中菌株A27-12最高,为70.59%,菌株A30-19抑制率最低为50.00%,两者呈显著差异。4株菌株的温室盆栽防效测定结果表明,菌株A30-19效果最好,其平均防效为78.79%,与58%甲霜灵锰锌800倍液的防效无显著差异,另3个菌株A36-15、A27-12和A20-8对烟草黑胫病的平均防效分别为67.95%、57.41%和50.46%。
239 actinomyces strains were isolated from 29 soil samples,of which 48 isolates had antagonistic effects on Phytophthora nicotianae with plate dual culture in proportion of 20.1 %.Based on the characteristics of morphology and physiology-biochemistry,the antagonists were identified as Streptomyces（47 strains,97.92 %）and Nocardia（1 strain）.4 actinomyces strains which showed superior antagonism in colony culture were selected to determine the inhibition of fermentation.The results showed that the average inhibition percentage was 59.80 %,of which strain A27-12 was the highest（70.59 %）,and the lowest inhibition percentage was A30-19（50.00 %）,and both had significant difference.The results of greenhouse test indicated that the 4 actinomycetes had different control effects on curing tobacco blank shank.The strain A30-19 had the best effect,78.79 %,which had no significant difference with 58 % Metalaxyl MnZn 800 times.Control effect of strain A36-15,A27-12 and A20-8 were 67.95 %,57.41 % and 50.46 %,respectively.
出处
《西南农业学报》
CSCD
北大核心
2012年第5期1708-1713,共6页
Southwest China Journal of Agricultural Sciences
基金
四川省烟草公司资助项目"四川省现代烟草农业建设中的植保系统工程建设"(08-123)
