摘要
【目的】有效防控烟草黑胫病。【方法】采用稀释分离法和平板对峙法从烟草种子中分离筛选拮抗烟草疫霉的内生菌,并通过发酵液抑菌、室内发芽、温室盆栽及漂浮育苗试验测定拮抗菌株对烟草幼苗的防病促生效果。【结果】从烟草种子中共计分离得到34株内生细菌,筛选出4株对烟草疫霉具有较强抑制作用的拮抗菌株,其中以YN201716的抑菌带最宽,YN201728的抑菌谱最广;温室盆栽试验结果表明:YN201728菌株发酵液灌根处理后,对烟草黑胫病的防效达58.84%。此外,YN201728菌株还促进种子萌发及烟草幼苗植株生长,与无菌水对照相比,幼苗的根长、株高、叶绿素、最大叶长、叶宽、鲜重和干重分别增加23.4%、83.3%、176.4%、124.6%、97.10%、487.8%和196.8%。结合形态特征、生理生化测试、16S rRNA基因序列测定及系统进化树分析的结果,YN201709、YN201728菌株为被鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),YN201702菌株为沙福芽孢杆菌(Bacillus safensis),YN201716菌株为短短芽孢杆菌(Brevibacillus brevis)。【结论】烟草种子内生菌YN201728菌株对烟草具有较好的防病促生效果,具有开发为微生物农药和菌肥的巨大潜力。
[Purpose]For tobacco black shank control purpose.[Method]The dilution plate and dual culture were proceeded to screen the tobacco seed endophytes activity against Phytophthora parasitica var.nicotiamae.The antagonistic and growth-promotion effects on tobacco seedlings were determined using disc filtrate assay,germination test,greenhouse experiment and floating seedling test.[Result]A total of 34 endophytic bacteria were isolated and4antagonistic strains with strong inhibitory effect on P.nicotianae.Among them,YN201716displayed maximum inhibitory zone,and YN201728 had the maximum inhibitory spectrum.The control effect of YN201728on tobacco black shank reached 58.84% under the greenhouse condition.Moreover,this strain showed a promotion effect on the seed germination and tobacco seedling growth.In addition,the root length,plant height,chlorophyll content,maximum leaf length,leaf width,fresh weight and dry weight of seedlings increase23.4%,83.3%,176.4%,124.6%,97.10%,487.8%and196.8%,respectively.Based on16S rRNA sequence alignment,YN201709and YN201728 strains were identified as Bacillus amyloliquefaciens,YN201702 as B.safensis,and YN201716 as Brevibacillus brevis.[Conclusion]The seedborne endophyte YN201728 displayed good antagonistic and growth-promoting effect on tobacco,and has great potential for development as microbial pesticide and bacterial fertilizer.
作者
焦蓉
刘剑金
杨焕文
何鹏飞
吴毅歆
王军伟
王戈
何月秋
JIAO Rong;LIU Jianjin;YANG Huanwen;HE Pengfei;WU Yixin;WANG Junwei;WANG Ge;HE Yueqiu(College of Agronomy and Biotechnology,Yunnan Agricultural University, Kunming 650201, China;Pu’er Branch of Yunnan Tobacco Company,Pu’er 665000, China;College of Tobacco Science,Yunnan Agricultural University,Kunming 650201, China;National Engineering Center for Applied Techniques ofAgricultural Biodiversity,Yunnan Agricultural University, Kunming 650201, China;Hongta Tobacco(Group) Co.,Ltd., Yuxi 653100, China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第6期1037-1045,共9页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省烟草公司科技计划项目(2015YN26).
关键词
烟草
烟草黑胫病
种子内生菌
生物防治
促生长
tobacco
Phytophthora parasitica var.nicotiamae
seedborne endophytic bacteria
biological control
growth-promoting
