摘要
【目的】对人类组织相容性抗原 (HumanLeucocyteAntigenSystem)Ⅱ类进行高分辨基因分析。【方法】用多重PCR和等位基因特异性PCR技术相结合[1,2 ] ,建立多重等位基因特异性PCR方法。【结果】 2 4对引物成功地分辨 43份DNA的HLA DRB1、B3、B4、B5 5 1个等位基因和DQB1 15个等位基因的 4位数特异性 ,识别基因片段长度为 2 81bp。【结论】多重等位基因特异性PCR技术敏感、微量、节省和分辨能力高 ,是进行复杂的HLA多态性研究中有价值的佐证试验。
Objectivel To analyse type ll HLA(Human Leucocyte Antigen) system genes in higher separative grades. Method By leaded multiplex PCR tecknique into allele specific PCR, the multiplex allele specific PCR(MAS PCR) method is estabilished. Results 43 DNA samples were typing on HLA DRB1,B3,B4,B5 and DQB1 seats by 24 primers, the identified gene fragment legth is 281 bp. Conclusions MAS PCR is sensitive, micro quantitative and save time. MAS PCR will be helpful on the DNA study of HLA system which has a large number of allele.
出处
《中山医科大学学报》
CSCD
2000年第3期179-181,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省自然科学基金资助课题!(960131)
