摘要
目的比较研究红芪替换经典补益方中的黄芪后对免疫抑制小鼠免疫功能的调节作用。方法小鼠腹腔注射环磷酰胺造成免疫抑制模型,以等量红芪替换补中益气和益气养血方剂中的黄芪,对模型小鼠灌胃治疗,测定胸腺指数、脾脏指数、淋巴细胞亚群、血清白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)的含量。结果用黄芪和用红芪的补中益气和益气养血方剂均能增加免疫抑制模型小鼠的胸腺和脾脏指数,差异有统计学意义(P<0.01),均能提高CD 19+、CD3+、CD4+和CD8+淋巴细胞百分比,差异有统计学意义(P<0.01),均能增加血清IL-4、IFN-γ的含量,差异有统计学意义(P<0.01),且在提高脾脏指数、CD3+淋巴细胞百分比和IFN-γ含量方面,用红芪的补中益气和益气养血组略高于应用黄芪的各组,差异具有统计学意义(P<0.01),其余指标两方剂用红芪与用黄芪之间比较,差异无统计学意义(P>0.05)。结论用黄芪和用红芪的补中益气和益气养血方剂均能增强环磷酰胺诱导的免疫抑制作用,且用红芪免疫调节作用稍强于相应用黄芪方剂。
Objective To compare and study the regulative effect of Hongqi （Radix Huangqi （Radix Astragali ） in classic tonifying and replenishing follnulas on i immunosuppressive mice. Methods The mouse model of immunosuppression intraperitoneal injection of cyclophosphamide （CTX）. The same dose of Huangqi was replaced by Hongqi in the formula of invigorating spleen and replenishing qi （Buzhongyiqi formula ） and formula of replenishing qi and tonifying blood （Yiqiyangxue formula）, and then the formulas were given to mice intragastrically. The thymus index, spleen index, lymphocyte subgroup and content of interleukin-4 （IL-4） and interferon-γ （IFN-γ） were detected. Results The formulas containing Huangqi or Hongqi all raised thymus index and spleen index in immunosuppressive mice （P 〈 0. 01 ）, increased the percentage of lymphocytes CD 19 ＋ , CD3 ＋ , CD4 ＋ and CD8 ＋ （P 〈0.01 ） , and increased the content of serum IL-4 and IFN-γ （ P 〈 0.01 ）. In the groups with Buzhongyiqi formula and Yiqiyangxue formulacontaining Hongqi, spleen index, lymphocyte CD3 ＋ and content of IFN-γwere slightly higher than those in the groups with the formulas containing Huangqi （P 〈 0.01 ）. The comparison in other indexes between the formulas containing Hongqi and those containing Huangqi showed that the difference was not statistically significant （ P 〉 0.05 ）. Conclusion The Buzhongyiqi formula and Yiqiyangxue formula containing Hongqi and those containing Huangqi all can improve the immunosuppressive effect of CTX, and Hongqi has higher immunoregulation etfect than Huangqi.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2012年第10期688-691,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
中国博士后科学基金资助项目(No.20090450017)
教育部高等学校博士点基金资助项目(No.200807330006)
关键词
红芪
黄芪
补中益气方剂
益气养血方剂
免疫抑制
淋巴细胞
小鼠
Hongqi （Radix Hedyseri）
Huangqi （Radix Astragali）
formulas of invigorating spleen and replenishing qi
formulas of replenishing qi and tonifying blood
immunosuppression
lymphocyte
mice
