摘要
目的研究微透析探针回收率与流速、药物浓度等之间的关系。方法以川芎嗪为检测药物,测定不同流速、不同药物浓度时,体外正、反透析法的探针回收率,以及体内反透析法的探针回收率。结果探针回收率与流速成反比,与川芎嗪浓度无关,体外正向与反向两种透析法得到的探针回收率差异较大。体外正向透析法与体内反向透析法得到的探针回收率比较接近。结论药动学研究中,可采用反向透析法测得的体内回收率作为药物的探针回收率。
ABSTRACT ： OBJECTIVE To explore the relationship between flow rate and drug concentration and the recoveries of microdialysis probes. METHODS Tetramethylpyrazine （TMP） was selected as the model drug. The in vitro recovery of TMP was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations. And the in vivo recovery of TMP was studied using retrodialysis method. RESULTS The microdialysis recoveries of TMP were inversely proportional to the perfusing rates while inde- pendent of TMP concentrations. The recoveries of positive dialysis were quite different from that of retrodialysis in vitro. While the recovery of in vitro positive dialysis was similar to that of in vivo retrodialysis . CONCLUSION Mierodialysis is a useful and reliable tool to study the pharmacokinetics of TMP and in vivo retrodialysis method can be used for TMP pharmacokinetics. KEY WORDS ： microdialysis recovery; microdialysis ; tetramethylpyrazine ; in vivo ; in vitro
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第21期1750-1753,共4页
Chinese Pharmaceutical Journal
基金
浙江中医局项目(2010ZA013)
浙江省医学重点学科群项目(XKQ-010-001)
浙江省科技计划项目(2011F10048)资助
关键词
探针回收率
微透析
川芎嗪
体内
体外
microdialysis recovery
microdialysis
tetramethylpyrazine
in vivo
in vitro
