摘要
目的探讨脑心舒胶囊的急性毒性及对小鼠抗应激能力的影响。方法测定小鼠灌胃给药的最大耐受量；将小鼠分为脑心舒胶囊高、中、低剂量组（2．4、1．2、0．6g／kg），阳性组（绞股蓝总苷片0．75g／kg），空白对照组，灌胃给药7d后，分别通过爬绳、负重游泳、耐缺氧、耐高／低温试验，观察脑心舒胶囊对抗应激能力的影响。结果脑心舒胶囊最大耐受量72g＆g，为药效学有效剂量的60倍以上。脑心舒胶囊高、中、低剂量组均可显著延长小鼠爬绳时间[分别为（9．5±3．1）min、（6．0±1．6）min、（4．1±1．2）min]、游泳耗竭时间[分别为（28．2±6．8）min、（25．7±10．3）min、（22．9±8．0）min]，与空白对照组[爬绳时间为（2．8±1．3）min，游泳耗竭时间为（15．7±7．0）mini比较差异有统计学意义（P〈0．05或P〈0．01）；脑心舒胶囊高、中剂量组可延长小鼠常压耐缺氧时的生存时间至17min（P〈0．05或P〈0．01）；脑心舒胶囊高剂量组可增强小鼠耐高／低温能力，死亡率降至30％（P〈0．05）。结论脑心舒胶囊能提高小鼠的抗应激能力，且安全性高。
Objective To investigate the acute toxicity and anti-stress effects of Naoxinshu capsule （NC） in mice. Methods In acute toxicity experiment, mice were oral administered to determine the maximum tolerance dose of NC. In anti-stress experiments, mice were divided into 5 groups at random, namely NC high, medium and low dose groups （2.4, 1.2 and 0.6 g/kg, respectively）, Gypenosides group （0.75 g/kg） and control group （n= 10）. After seven days of oral administration, rope climbing time, loaded swimming time, anti-hypoxia time, and survival rate under high or low temperature tests were carded out, respectively. Results The maximum tolerance dose of NC was 72 g/kg. NC high, medium and low dose groups significantly prolonged the rope climbing time [is （9.5±3.1） min, （6.0 ±1.6） min, （4.1± 1.2） rain], loaded swimming time [is （28.2 ±6.8） rain, （25.7 ± 10.3） min, （22.9 ±8.0） mini and anti-hypoxia time （around 17 min）, compared to the control group （P〈 0.05 or P（0.01）. NC high dose group significantly improved the survival rate of mice （i.e. 30%） under high or low temperature （P〈0.05）. Conclusion NC shows good anti-stress effects, and no detectable acute toxicity in mice.
出处
《国际中医中药杂志》
2012年第11期994-996,共3页
International Journal of Traditional Chinese Medicine
关键词
脑心舒胶囊
小鼠
急性毒性
抗应激
Naoxinshucapsule
Mouse Acute toxicity
Anti-stress
