摘要
目的:观察以补肾益脾为治则的古汉养生精对力竭运动小鼠血乳酸、乳酸脱氢酶和运动时间的影响,以及对抗运动性疲劳的作用。方法:实验于2004-04/05在湛江师范学院中心实验室和体育系实验室完成。①4周龄昆明种健康小鼠30只,雌雄各半,体质量(32.80±2.42)g,按完全随机数字方法分为对照组,运动组,古汉养生精组,每组10只。古汉养生精片(购自清华紫光古汉生物制药有限公司,国药准字Z10970128,由淫羊藿、黄精、黄芪、枸杞等组成,含生药2g/片),蒸馏水溶解稀释成10%溶液。②运动组和古汉养生精组每天进行游泳1次,6d/周,共4周,第1周每天游泳1h,第2周每天游泳1.5h,第3,4周每天游泳2h。③古汉养生精组每天按3g/kg体质量的古汉养生精灌胃,运动组以等体积生理盐水灌胃,对照组不作任何处理。④4周后记录小鼠游泳力竭时间和血乳酸含量、血浆乳酸脱氢酶活性、超氧化物歧化酶活性和丙二醛含量。结果:①小鼠游泳力竭时间:运动组小鼠游泳力竭时间显著短于古汉养生精组眼(347.69±61.66),(438.68±62.86)min,P<0.01演。②血乳酸含量、乳酸脱氢酶活性、丙二醛含量:运动组显著高于古汉养生精组(926.81±84.20)比(659.45±73.35)mmol/L,P<0.01;(194.215±5.727)比(162.179±7.400)μkat/L,P<0.05;(15.46±0.46)比(9.04±0.93)μmol/L,P<0.01演。③血浆超氧化物歧化酶活性:运动组显著低于古汉养生精组眼(1.480±0.268),(2.276±0.273)μkat/L,P<0.01演。结论:服用古汉养生精可以延长小鼠力竭运动时间,减轻运动性疲劳的产生和自由基介导的脂质过氧化反应。
AIM： To observe the effects of ancient han health-keeping （AHHK） extract on blood lactate （La）, lactate dehydrogenate （LDH） and movement time of exhaustive exercise mice, and study the anti-fatigue effects of AHHK. METHODS： The experiment was conducted at the Central Laboratory and Laboratory of Physical Education Department, Zhanjiang Normal University from April to May in 2004. ①Thirty Kunming mice of 4 weeks old and （32.80±2.42） g without gender limitation were divided randomly into three groups, namely control group, training group and AHHK group, with 10 mice in each. AHHK extract （purchased from Tsinghua Unisplendour Guhan Bio-pharmaceutical Corporation Ltd., No.Z10970128, consisting of Herba Epimedii, siberian solomonseal rhizome, mongolian milkvetch root and medlar, etc., 2 g crude drug for a tablet） was diluted into 10% solution by distilled water. ②The swimming training was performed in the mice of training group and AHHK group, once daily, 6 days a week for total 4 weeks, and 1 hour in the first week, 1.5 hours in the second week and 2 hours in the third and fourth weeks. ③Everyday the mice of AHHK group and training group were injected intragastrically with 3 g/kg AHHK extract and equal saline respectively, while the control mice received no management.④After four weeks, the mice＇s exhaustive swimming time, La content, blood plasma LDH activity, super oxide dismutase （SOD） activity and malondialdehyde （MDA） content were determined after exhaustive swimming. RESULTS： ①The exhaustive swimming time of mice： The time of AHHK group was significantly longer than that of training group [（347.69±61.66）, （438.68±62.86） minutes, P 〈 0.01].②The La content, LDH activity and MDA content： They were all increased significantly in training group, compared with AHHK group [（962.81±84.20） mmoL/L, （659.45±73.35） mmol/L, P 〈 0.01; （194.215±5.272） μkat/L, （162.179±7.400） μkat/L, P 〈 0.05; （15.46±0.46） μmol/L, （9.04±0.93） μmol/L, P 〈 0.01],③Blood plasma SOD activity of training group was significantly lower than those of AHHK group [（1.480±0.268）, （2.276±0.273） μkat/L, P 〈 0.01 ]. CONCLUSION： AHHK extract administration can prolong the exhaustive exercise time of the moving mice, and reduce the appearance of sports fatigue as well as lipid peroxidation mediated by free radicals.
出处
《中国临床康复》
CSCD
北大核心
2006年第35期95-97,共3页
Chinese Journal of Clinical Rehabilitation
