摘要
目的:通过研究无患子皂苷对自发性高血压大鼠(spontaneously hypertensive rats,SHR)离体主动脉血管对于不同血管收缩剂和舒张剂反应性以及对血管内皮活性物质血清含量的影响,考察无患子皂苷对SHR内皮功能的调节作用,为进一步研究其作用及机制提供实验依据。方法:取16周龄SHR 40只,随机均分为5组,即SHR模型组、阳性对照组(卡托普利,27 mg·kg-1)、无患子皂苷低、中、高(27,54,108 mg·kg-1)剂量组,另取8只健康WKY大鼠作为正常对照组,按剂量连续给药8周,检测指标如下:①对胸主动脉血管对于不同血管收缩剂KCl(20～120 mmol·L-1),PE(1×10-8～1×10-4mol·L-1),AngⅡ(1×10-9～10-5mol·L-1)反应性的影响;②对Ach(1×10-10～1×10-5mol·L-1)血管内皮依赖性及SNP(1×10-8～1×10-3mol·L-1)非内皮依赖性血管舒张反应的影响;③ELISA法检测对血管活性物质NO,6-KPG1α,ET-1,TXB2血清含量的影响。结果:SHR模型组内皮损伤、功能失衡,表现为主动脉环对KCl,PE,AngⅡ诱发的血管收缩反应性增强,对Ach诱发的内皮依赖性舒张反应减弱,而对SNP诱发的非内皮依赖性舒张反应未见明显变化;其血清TXB2,ET-1含量增加,而NO,6-KPG1α含量减少;无患子皂苷各剂量组可抑制主动脉对KCl,PE,AngⅡ的收缩反应,增强对Ach的内皮依赖性舒张效应,降低血清TXB2,ET-1含量,增加NO,6-KPG1α含量,上述指标与SHR对照组有显著性差异(P<0.05或P<0.01)。结论:无患子皂苷对SHR内皮功能具有一定的保护作用,其机制与恢复血管内皮活性物质TXB2,ET-1/NO,6-KPG1α平衡状态有关。
Objective： To investigate the regulation on endothelial function of sapindus saponins in spontaneously hypertensive rats by studying the reactivity on different vasoconstrictor and dilator, and the content of the active substances. Method： Forty 16-week- old spontaneously hypertensive rats were randomly divided into five groups, one with placebo as model group, one with captopril tablets （27 mg · kg^- 1 ）as positive control, one with low-dose sapindus saponins （27 mg mg · kg^- 1 ）, one with medium-dose （54 mg mg · kg^- 1 ）, one with high-dose （108 mg mg · kg^- 1 ）. And another eight healthy Wistar-Kyoto strain（WKY） rats were used as the normal group. The animals were treated for eight weeks, and the indicators to be detected were as follows： （1） the response of thoracic aorta on different vasoconstrictors Ang I1 （ 1 × 10 ^-9-1 × 10^-5mol · L^-1 ） ,PE（ 1 × 10^-8-1 × 10^-4 mol . L^-1 ） ,KC1（20 -120 mmol · L^-1 ） ; （2）the endothelium-dependent or non-endothelium-dependent vasodilation response of horacic aorta on Ach（1 × 10^-10-1 × 10^-5 mol · L^-1） or SNP （ 1 × 10^-8-1 × 10^-3 mol ·L^-1 ） ; （3） the content of NO,6-KPGlo, ET-1 and TXB2 in serum was determined by Elisa. Result： In SHR model group, the response of thoracic aorta on Ang 11 , PE and KC1 was increased, the eudothelium-dependent vasodilation on Ach was reduced, but the effects on SNP was not obvious, the content of ET-1 and TXB2was increased, and the content of NO and 6-KPG1α was reduced, Vs the normal control group, there were significant differences （P 〈 0. 05 or P 〈 0. 01 ） ; in the treatment groups, the response of thoracic aorta on Ang II , PE and KC1 was reduced, the endothellum-dependent vasodilation of boracic aorta on Ach was in- proved, the content of ET-1 and TXB2 was reduced, and the content of NO and 6-KPG1α was increased, Vs the SHR model group, there were significant differences （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： Our findings suggested that sapindus saponins protected the endothelial function in SHR, the mechanisms were relevant to the protection of endothelial function.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第21期3282-3287,共6页
China Journal of Chinese Materia Medica
基金
安徽省教育厅高等学校优秀青年人才基金项目(2009SQRZ114)
安徽中医学院青年科学研究基金项目(2012QN005)
关键词
无患子皂苷
自发性高血压
内皮功能
sapindus saponins
spontaneously hypertension
endothelial function
