摘要
[Objective] This study aimed to clone and identify the banana fruit receptor-like protein kinase gene.[Method] The cDNA phage libraries of banana fruit were adopted as the experimental materials to screen positive phage libraries of banana receptor-like protein kinase gene;cloning and sequence analysis of the gene were conducted,and the banana receptor-like protein kinase gene was identified by using in situ hybridization method.[Result] In this study,a 1 698 bp long banana receptor-like protein kinase gene was cloned from banana fruit,encoding 563 amino acids.Southern hybridization result confirmed that the banana receptor-like protein kinase gene was a multiple-copy gene from banana genome.[Conclusion] The study laid the foundation for further investigating the functions of banana receptor-like protein kinase gene in fruit.
[目的]克隆鉴定香蕉类受体蛋白激酶基因。[方法]以香蕉果实cDNA噬菌体文库为材料,筛选出香蕉类受体蛋白激酶基因阳性噬菌体库,对该基因进行克隆和序列分析,并通过原位杂交方法对其进行鉴定。[结果]试验克隆到1个长度为1698bp的香蕉果实类受体蛋白激酶基因,编码563个氨基酸序列。经过Southern杂交证实该基因来自香蕉基因组,是1个多拷贝基因。[结论]该研究结果为进一步研究香蕉类受体蛋白激酶基因在香蕉果实中的功能奠定了基础。
基金
Supported by National Science and Technology Project in Rural Areas in the 12th Five-year Plan(2011AA10020605)
Natural Science Foundation of Hainan Province(809038)
Special Fund for Basic Research and Operating Expenses of Central Nonprofit Research Institutes(ITBB110209,1630052012003)
Key Project of Science and Technology in Hainan Province(ZDXM20120024)~~
