摘要
【 目的建立反相高效液相色谱测定血浆中茶碱浓度的方法。方法以ODS—C18柱为固定相，甲醇：水（32：68）为流动相；流速0．8ml／min；柱温35℃；检测波长275nm，测定茶碱的血药浓度。血浆中的茶碱用氯仿-异丙醇（95：5）提取。结果茶碱在4．0—24．00μg／ml浓度范围内呈良好线性关系，r=0．9994；平均回收率为99．06％；日内和日间RSD均小于2．0％。结论本法灵敏简便、准确可靠，可作为茶碱血药浓度监测的常规方法。
Objective To establish a method of RP - HPLC for the determination of theophylline concentration in human plasma. Methods RP - HPLC was carried out on Yilite - C18. The mobile phase was composed of metha- nol -water（32：68 ）, the flow rate was l. 0 ml/min, the column temperature was set at 35℃, the detecting wavelength was set at 275nm. The wavelength for detection was 275 nm. The theophylline was extracted from plasma using a mix- ture of trichloromethane ： isopropanol （ 95 ： 5, V/V）. Results The assay was shown to produce linear calibration curves over the range from 4.0 - 24.00μg/ml for theophylline, r = 0. 9997. The average recovery was 99.06% and the inter - day RSD and intra - day RSD of theophylline were less than 2.0%. Conclusion The method is found to be sensi- tive, simple, convenient＇and accurate for the determination of theophylline concentration in human plasma. It also can be used as a routine method by TDM.
出处
《医学新知》
CAS
2012年第5期338-340,共3页
New Medicine
