摘要
目的研究CD117、Smoothelin在重度慢传输型便秘（slowtransitconstipation，STC）患者病变结肠中的表达，探讨其在STC发病机制中的可能作用。方法采用免疫组化法，检测30例STC患者和20例对照组结肠组织中CD117、Smoothelin的分布及表达水平，并进行图像分析半定量分析比较。结果CD117主要分布于结肠黏膜下层、肌层，其中以肌间神经丛明显；Smoothelin主要分布于黏膜下肌层、环肌层和纵肌层。半定量结果显示：与对照组比较，STC结肠组织中CD117、Smoothelin表达明显降低。结论STC患者结肠中CD117、Smoothelin的表达下调，提示Cajat间质细胞和平滑肌细胞可能参与了STC的发病机制；临床上可将CD117和Smoothelin作为STC分子病理诊断辅助指标。
Objective To investigate the CD117 and Smoothelin expressions in the colonic tissues from patients with severe slow transit constipation （STC）, and explore its possible role in the pathogenesis of STC. Methods We Used envision immunohistochemical staining and detected the distribution and expression levels of CD117 and Smooth- elin in the colon of 30 STC patients and 20 cases in the control group. Results CD117 was mainly distributed in the submucosa and muscular layer. Smoothelin was mainly distributed in the colonic submucosa muscular layer, circular muscular layer and longitudinal muscular layer. The semi -quantitative results showed that the expressions of CD117 and Smoothelin in STC colonic tissue were significantly reduced compared with the control group. Conclusion The expressions of CD117 and Smoothelin were down - regulated in the colon of patients with STC. The present study indi- cates that interstitial cells of Cajal（ICC） and mooth muscle cell may be involved in the pathogenesis of STC.
出处
《医学新知》
CAS
2012年第5期331-332,F0004,共3页
New Medicine
基金
基金项目：湖北省科学技术研究和开发项目（编号417）
湖北省自然科学基金项目（编号2011CDB521）
