摘要
目的:探讨针刺治疗脑梗死介入时机及脑缺血再灌注损伤与炎性细胞因子受体的关系。方法:采用雄性健康Wistar大鼠110只,随机分为正常组10只、假手术组10只、模型组10只、非穴位针刺组40只及醒脑开窍针刺组40只。其中两个针刺组内再设缺血1h再灌注1h组、3h组、6h组、12h组4个亚组,每个亚组10只大鼠。两个针刺组分别于缺血再灌注1h、3h、6h、12h4个时间点进行针刺干预,醒脑开窍针刺组穴取"水沟""内关";非穴位针刺组取双胁下非穴点。在相应时间点断头取脑,采用实时定量聚合酶链反应及免疫印迹法检测白细胞介素-1受体(IL-1RⅠ)与肿瘤坏死因子-α受体(TNFR-Ⅰ)mRNA和蛋白变化。结果:模型组大鼠IL-1RⅠ、TN-FR-ⅠmRNA和蛋白较正常组、假手术组、醒脑开窍针刺组、非穴位针刺组明显上升(P<0.01,P<0.05);醒脑开窍针刺组内IL-1RⅠ、TNFR-ⅠmRNA和蛋白在缺血1h再灌注3h表达最弱,其次为6h、1h、12h;非穴位针刺组内IL-1RⅠ、TNFR-ⅠmRNA和蛋白各时间点间表达无显著差异,但各时间点与模型组比较有所下降(均P<0.05)。结论:针刺可降低脑缺血再灌注IL-1RⅠ和TNFR-Ⅰ的mRNA和蛋白的表达,抑制致炎因子受体过表达而阻断凋亡信号的传导,延长脑缺血时间治疗窗,从而起到脑保护作用。在缺血超早期3h介入针刺治疗为最佳时间。
Objective To explore the intervention timing of acupuncture in treatment of cerebral infarction and the relationship of cerebral ischemia reperfusion injury with inflammatory cytokine receptor.Methods One hund-red and ten male healthy Wistar rats were randomly divided into a normal group（n=10）,a sham operation group（n=10）,a model group（n=10）,an acupuncture at non-acupoint group（non-acupoint group,n=40）,an acupuncture with regaining consciousness method group（regaining consciousness group,n=40）.Four subgroups were set up 1 h ischemia reperfusion in 1 h group,3 h group,6 h group,12 h group in the two acupuncture groups,10 rats in each subgroup.Two acupuncture groups were treated with acupuncture at four time points（1 h,3 h,6 h and 12 h after ischemia reperfusion）,and ＂Shuigou＂（GV 26） and ＂Neiguan＂（PC 6） were selected in regaining consciousness group,and the non-acupoints below the bilateral costal region were selected in non-acupoint group.At the corresponding time point,the tissues of the brain were removed and interleukin1 receptor（IL-1RⅠ） and tumor necrosis factor receptor（TNFR-Ⅰ） mRNA and protein changes were detected by using real-time quantitative polymerase chain reaction and immunoblot assay.Results The expression of IL-1RⅠ and TNFR-Ⅰ mRNA and protein in the model group were significantly higher than that in normal group,sham operation group,regaining consciousness group and non-acupoint group（P0.01,P0.05）.The expression of IL-1RⅠ and TNFR-Ⅰ mRNA and protein in regaining consciousness group was weakest at 3 h after reperfusion followed successively by 6 h,1 h,12 h,and there was no significantly change of IL-1RⅠ and TNFR-Ⅰ mRNA and protein expression in non-acupoint group among different timing points,but which was decreased as compared with those in the model group at the same time point（all P0.05）.Conclusion Acupuncture can reduce the expression of IL-1RⅠ and TNFR-Ⅰ mRNA and protein in rats with cerebral ischemia reperfusion,inhibit the excessive expression of proinflammatory cytokine receptor,block apoptosis signal transduction and extend time window for treatment of cerebral ischemia,so as to play the protective effect for brain.Within 3 h of ischemia is the best time for intervention of acupuncture treatment.
出处
《中国针灸》
CAS
CSCD
北大核心
2012年第11期1012-1018,共7页
Chinese Acupuncture & Moxibustion
基金
江苏省中医药领军人才科研项目:LJ200918
关键词
脑缺血再灌注
醒脑开窍
炎性细胞因子受体
Brain Ischemia Reperfusion
Consciousness Restor Resuscitat
Inflammatory Cell Factor Receptor
