摘要
目的探讨脑缺血肺组织白细胞介素-6(IL-6)的表达变化。方法将成年SD大鼠随机分为假手术组和脑缺血肺损伤组,用线栓法建立大脑中动脉堵塞脑缺血肺损伤模型。脑缺血后24h取肺组织用RT-PCR,48h用Western blot技术检测肺组织IL-6的表达量。脑缺血后72h用免疫组织化学技术检测IL-6蛋白定位分布。结果肺组织IL-6基因和蛋白表达在脑缺血肺组织明显增加,与对照组比较,差异有统计学意义(P<0.05)。IL-6阳性染色主要位于气道上皮细胞胞和巨噬样细胞。结论脑缺血后肺组织IL-6表达明显上调,提示IL-6与脑缺血肺损伤有一定关系。
Objective To study the expression changes of interleukin-6 （IL-6） in the lung of rats with brain ischemia. Methods Adult SD rats were divided into sham operation group and brain ischemia lung injury （BILI） group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. RT-PCR was applied to detect the IL-6 expression level of lung at 24 h after brain ischemia. The IL-6 protein in lung at 48 h after brain ischemia was detected with Western blot. Immunohistochemistry method was used to demonstrate the IL-6 protein location in lung at 72 h after brain ischemia. Results Increased immunostaining, protein level and mRNA expression for IL-6 were found in lung at investigated time point after brain ischemia. Conclusion IL-6, as a crucial preinflammatory factor, could be related to airway inflammation in rats induced by brain ischemia.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2012年第6期907-909,917,共4页
Journal of Sichuan University(Medical Sciences)
基金
云南省自然科学基金(No.2010ZC206,No.2011FZ272)资助
