摘要
目的探讨大鼠局灶性脑缺血钙离子分布的时空变化及葛根素对钙超载的保护作用。方法采用线栓法制备大鼠大脑中动脉局灶性脑缺血模型,在缺血后不同时间点应用葛根素进行干预,运用TTC染色观察脑梗死体积的变化,利用激光共聚焦显微镜观察局灶性脑缺血后不同时间点活脑片皮层及纹状体Ca2+的动态变化及其分布。结果缺血后2 h组梗死侧皮层及纹状体Ca2+荧光强度均开始升高,并随缺血时间的延长而进一步增强(P<0.001);假手术组皮层与纹状体Ca2+荧光强度无明显差异,而缺血后梗塞侧纹状体的Ca2+荧光强度明显高于皮层。与模型对照组各对应时间点相比,模型干预组2 h组、12 h组大鼠脑皮层和纹状体Ca2+荧光强度均明显下降,差异显著(P<0.05),24 h组Ca2+荧光强度虽有所下降,但尚无统计学意义。结论葛根素可能是通过减轻钙超载从而挽救半暗带,减小脑梗死体积,发挥神经保护作用的;12 h可能为葛根素治疗缺血性脑损伤的时间窗。
Objective To observe the temporal and spatial changes in the distribution of Ca2＋ in the rat brain following focal cerebral ischemia injury and explore the protective effect of puerarin against calcium overload.Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion in rats.After cerebral ischemia,puerarin was administered in the rats at different time points.The volume of ischemic cerebral tissue was assessed by TTC staining,and the fluorescence intensity of Ca2＋ in the cortex and corpora striata was determined under laser scanning confocal microscope.Results The fluorescence intensity of Ca2＋ in the infracted cortex and corpora striata begun to increase 2 h after the ischemia and was further enhanced with the prolongation of the ischemic time.No significance was found in the fluorescence intensity of Ca2＋ between the cortex and corpora striata.The fluorescence intensity of Ca2＋ in the infarcted corpora striata was obviously higher than that in the cortex after ischemia.Compared with that in the ischemic model group,the fluorescence intensity of Ca2＋ in the infarcted cortex and corpora striata decreased significantly at 2 and 12 h following puerarin intervention（P0.05）.Conclusion Puerarin treatment can relieve calcium overload,reduce cerebral ischemic volume and play a neuroprotective role against focal cerebral ischemia.Twelve hours following cerebral ischemic injury may be the time window for administering puerarin intervention.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第6期1268-1271,共4页
Journal of Southern Medical University
基金
陕西省科学技术研究发展计划(2006K16-G5(3))
关键词
葛根素
钙超载
激光扫描共聚焦显微镜
脑缺血
puerarin
calcium overload
laser scanning confocal microscope
cerebral ischemia
